Difference between revisions of "LytD"

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* '''Mutant:'''
 
* '''Mutant:'''
 
** 1A788 ( ''lytD''::''tet''), {{PubMed|7934877}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A788&Search=1A788 BGSC]
 
** 1A788 ( ''lytD''::''tet''), {{PubMed|7934877}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A788&Search=1A788 BGSC]
 +
** 1A792 ( ''lytD''::''tet''), {{PubMed|1588906}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A792&Search=1A792 BGSC]
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 12:29, 19 September 2012

  • Description: glucosaminidase

Gene name lytD
Synonyms cwlG
Essential no
Product glucosaminidase
Function major autolysin, cell separation
Gene expression levels in SubtiExpress: lytD
MW, pI 95 kDa, 9.935
Gene length, protein length 2640 bp, 880 aa
Immediate neighbours tagC, pmi
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LytD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
LytD expression.png
























Categories containing this gene/protein

cell wall degradation/ turnover

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

  • Locus tag: BSU35780

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • A mutation was found in this gene after evolution under relexed selection for sporulation PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure (according to Swiss-Prot)
  • Protein family: glycosyl hydrolase 73 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Christopher T Brown, Laura K Fishwick, Binna M Chokshi, Marissa A Cuff, Jay M Jackson, Travis Oglesby, Alison T Rioux, Enrique Rodriguez, Gregory S Stupp, Austin H Trupp, James S Woollcombe-Clarke, Tracy N Wright, William J Zaragoza, Jennifer C Drew, Eric W Triplett, Wayne L Nicholson
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Appl Environ Microbiol: 2011, 77(19);6867-77
[PubMed:21821766] [WorldCat.org] [DOI] (I p)

Rui Chen, Sarah B Guttenplan, Kris M Blair, Daniel B Kearns
Role of the sigmaD-dependent autolysins in Bacillus subtilis population heterogeneity.
J Bacteriol: 2009, 191(18);5775-84
[PubMed:19542270] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

P Margot, C Mauël, D Karamata
The gene of the N-acetylglucosaminidase, a Bacillus subtilis 168 cell wall hydrolase not involved in vegetative cell autolysis.
Mol Microbiol: 1994, 12(4);535-45
[PubMed:7934877] [WorldCat.org] [DOI] (P p)