Difference between revisions of "ArgF"

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|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of arginine
 
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of arginine
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU11250 argF]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11250 argF]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[carB]]'', ''[[yjzC]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[carB]]'', ''[[yjzC]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12966&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU11250 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU11250 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU11250 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:argF_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:argF_context.gif]]

Revision as of 12:31, 13 May 2013

  • Description: ornithine carbamoyltransferase

Gene name argF
Synonyms
Essential no
Product ornithine carbamoyltransferase
Function biosynthesis of arginine
Gene expression levels in SubtiExpress: argF
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 34 kDa, 4.965
Gene length, protein length 957 bp, 319 aa
Immediate neighbours carB, yjzC
Sequences Protein DNA Advanced_DNA
Genetic context
ArgF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ArgF expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

AhrC regulon

The gene

Basic information

  • Locus tag: BSU11250

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline (according to Swiss-Prot)
  • Protein family: ATCase/OTCase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2EF0 (from Thermus thermophilus hb8, 48% identity, 63% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

A Mountain, N H Mann, R N Munton, S Baumberg
Cloning of a Bacillus subtilis restriction fragment complementing auxotrophic mutants of eight Escherichia coli genes of arginine biosynthesis.
Mol Gen Genet: 1984, 197(1);82-9
[PubMed:6096675] [WorldCat.org] [DOI] (P p)