YxeN

• Description: putative cysteine ABC transporter (permease)
  
  

Gene name	yxeN
Synonyms
Essential	no
Product	putative cysteine ABC transporter (permease)
Function	uptake of cysteine
Gene expression levels in SubtiExpress: yxeN
Interactions involving this protein in SubtInteract: YxeN
MW, pI	24 kDa, 9.484
Gene length, protein length	672 bp, 224 aa
Immediate neighbours	yxeO, yxeM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, biosynthesis/ acquisition of amino acids, membrane proteins

This gene is a member of the following regulons

CymR regulon

The gene

Basic information

• Locus tag: BSU39490

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: binding-protein-dependent transport system permease family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: YxeO-YxeN-YxeM PubMed

• Localization: cell membrane PubMed

Database entries

• Structure:

• UniProt: P54953

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yxeK-yxeL-yxeM-yxeN-yxeO-yxeP-yxeQ PubMed

• Expression browser: yxeN PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of cysteine (CymR) PubMed

• Regulatory mechanism:
  • CymR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A950 ( yxeN::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Sergine Even, Pierre Burguière, Sandrine Auger, Olga Soutourina, Antoine Danchin, Isabelle Martin-Verstraete
Global control of cysteine metabolism by CymR in Bacillus subtilis.
J Bacteriol: 2006, 188(6);2184-97
[PubMed:16513748] [WorldCat.org] [DOI] (P p)

Ken-Ichi Yoshida, Izumi Ishio, Eishi Nagakawa, Yoshiyuki Yamamoto, Mami Yamamoto, Yasutaro Fujita
Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome.
Microbiology (Reading): 2000, 146 ( Pt 3);573-579
[PubMed:10746760] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)