Difference between revisions of "GanA"
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|style="background:#ABCDEF;" align="center"|'''Gene name''' | |style="background:#ABCDEF;" align="center"|'''Gene name''' | ||
| − | |'' | + | |''ganA'' |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yvfN '' | + | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yvfN, lacA '' |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
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|style="background:#ABCDEF;" align="center"|'''Function''' || galactan utilization | |style="background:#ABCDEF;" align="center"|'''Function''' || galactan utilization | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU34130 | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU34130 ganA] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 79 kDa, 5.704 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 79 kDa, 5.704 | ||
| Line 20: | Line 20: | ||
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2061 bp, 687 aa | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2061 bp, 687 aa | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ | + | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ganB]]'', ''[[ganQ]]'' |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15418]+-newId sequences] <br/> (Barbe ''et al.'', 2009)''' | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15418]+-newId sequences] <br/> (Barbe ''et al.'', 2009)''' | ||
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__TOC__ | __TOC__ | ||
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<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
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<br/><br/> | <br/><br/> | ||
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | * a ''[[cycB]]-[[ganP]]-[[ganQ]]-[[ganA]]-[[ganB]]'' mutant is impaired in the utilization of galactan {{PubMed|22893383}} | ||
=== Database entries === | === Database entries === | ||
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=== Additional information=== | === Additional information=== | ||
| − | |||
| − | |||
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=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' | + | * '''Catalyzed reaction/ biological activity:''' |
| + | ** degradation of galactotriose to galactose {{PubMed|22893383}} | ||
* '''Protein family:''' glycosyl hydrolase 42 family (according to Swiss-Prot) | * '''Protein family:''' glycosyl hydrolase 42 family (according to Swiss-Prot) | ||
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' ''[[cycB]]-[[ | + | * '''Operon:''' ''[[cycB]]-[[ganP]]-[[ganQ]]-[[ganA]]-[[ganB]]'' {{PubMed|9287030}} |
| − | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ganA_3503020_3505083_-1 | + | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ganA_3503020_3505083_-1 ganA] {{PubMed|22383849}} |
* '''Sigma factor:''' | * '''Sigma factor:''' | ||
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=References= | =References= | ||
| − | <pubmed>2104611,9287030,17056685 , 22517742</pubmed> | + | <pubmed>2104611,9287030,17056685 , 22517742 22893383</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 13:53, 16 August 2012
- Description: beta-galactosidase
| Gene name | ganA |
| Synonyms | yvfN, lacA |
| Essential | no |
| Product | beta-galactosidase |
| Function | galactan utilization |
| Gene expression levels in SubtiExpress: ganA | |
| MW, pI | 79 kDa, 5.704 |
| Gene length, protein length | 2061 bp, 687 aa |
| Immediate neighbours | ganB, ganQ |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
utilization of specific carbon sources, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU34130
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- degradation of galactotriose to galactose PubMed
- Protein family: glycosyl hydrolase 42 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- phosphorylated on Arg-387 PubMed
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: O07012
- KEGG entry: [3]
- E.C. number: 3.2.1.23 3
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- Additional information:
Biological materials
- Mutant:GP1181 (aphA3) available in the Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Yunrong Chai, Pascale B Beauregard, Hera Vlamakis, Richard Losick, Roberto Kolter
Galactose metabolism plays a crucial role in biofilm formation by Bacillus subtilis.
mBio: 2012, 3(4);e00184-12
[PubMed:22893383]
[WorldCat.org]
[DOI]
(I e)
Alexander K W Elsholz, Kürsad Turgay, Stephan Michalik, Bernd Hessling, Katrin Gronau, Dan Oertel, Ulrike Mäder, Jörg Bernhardt, Dörte Becher, Michael Hecker, Ulf Gerth
Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis.
Proc Natl Acad Sci U S A: 2012, 109(19);7451-6
[PubMed:22517742]
[WorldCat.org]
[DOI]
(I p)
Stephanie Shipkowski, Jean E Brenchley
Bioinformatic, genetic, and biochemical evidence that some glycoside hydrolase family 42 beta-galactosidases are arabinogalactan type I oligomer hydrolases.
Appl Environ Microbiol: 2006, 72(12);7730-8
[PubMed:17056685]
[WorldCat.org]
[DOI]
(P p)
R A Daniel, J Haiech, F Denizot, J Errington
Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR.
J Bacteriol: 1997, 179(17);5636-8
[PubMed:9287030]
[WorldCat.org]
[DOI]
(P p)
J Errington, C H Vogt
Isolation and characterization of mutations in the gene encoding an endogenous Bacillus subtilis beta-galactosidase and its regulator.
J Bacteriol: 1990, 172(1);488-90
[PubMed:2104611]
[WorldCat.org]
[DOI]
(P p)
