OpuE

• Description: proline transporter
  
  

Gene name	opuE
Synonyms	yerK
Essential	no
Product	proline transporter
Function	compatible solute transport
Gene expression levels in SubtiExpress: opuE
Metabolic function and regulation of this protein in SubtiPathways: Pro, Stress
MW, pI	53 kDa, 9.815
Gene length, protein length	1476 bp, 492 aa
Immediate neighbours	sapB, gatC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

general stress proteins (controlled by SigB), transporters/ other, coping with hyper-osmotic stress, membrane proteins

This gene is a member of the following regulons

SigB regulon, CcpA regulon

The gene

Basic information

• Locus tag: BSU06660

Phenotypes of a mutant

• strong growth disadvantage in high-salinity minimal media lacking proline PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: solute symporter family

• Paralogous protein(s): PutP

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O06493

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: opuE PubMed

• Sigma factor: SigB PubMed

• Regulation:
  • induced by stress (SigB) PubMed
  • induced by glucose (CcpA) PubMed

• Regulatory mechanism:
  • CcpA: transcription activation PubMed

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Erhard Bremer, University of Marburg, Germany homepage