Difference between revisions of "PcrA"

From SubtiWiki
Jump to: navigation, search
Line 36: Line 36:
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
 
 
 
<br/><br/>
 
<br/><br/>
  
Line 91: Line 87:
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
 
+
** unwinding of the double-stranded ICEBs1 DNA is stimulated by [[HelP]] {{PubMed|23326247}}
 
* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
 
** PcRA interacts with the [[RNA polymerase]] {{PubMed|21710567}}
 
** PcRA interacts with the [[RNA polymerase]] {{PubMed|21710567}}
Line 118: Line 114:
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pcrA_719370_721589_1 pcrA] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pcrA_719370_721589_1 pcrA] {{PubMed|22383849}}
  
* '''Sigma factor:'''  
+
* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
Line 151: Line 147:
 
==Original Publications==
 
==Original Publications==
 
'''Additional publications:''' {{PubMed|21710567}}
 
'''Additional publications:''' {{PubMed|21710567}}
<pubmed>12073041,9701819 ,16267290, 19943900 20723756 18276648 17449621 10899133 10199404 </pubmed>
+
<pubmed>12073041,9701819 ,16267290, 19943900 20723756 18276648 17449621 10899133 10199404 23326247 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 14:44, 13 February 2013

  • Description: ATP-dependent DNA helicase, facilitates unwinding of ICEBs1 DNA for horizontal transfer

Gene name pcrA
Synonyms yerF
Essential yes PubMed
Product ATP-dependent DNA helicase
Function plasmid rolling-circle replication, conjugative transfer of ICEBs1
Gene expression levels in SubtiExpress: pcrA
Interactions involving this protein in SubtInteract: PcrA
MW, pI 83 kDa, 5.767
Gene length, protein length 2217 bp, 739 aa
Immediate neighbours pcrB, ligA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PcrA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PcrA expression.png















Categories containing this gene/protein

DNA repair/ recombination, mobile genetic elements, essential genes

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

  • Locus tag: BSU06610

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • PcrA anchors itself to the template duplex using the 2B subdomain and reels in the lagging strand, extruding a single-stranded loop PubMed
    • dismantles RecA filaments PubMed
  • Protein family: uvrD-like helicase C-terminal domain (according to Swiss-Prot)
  • Paralogous protein(s): YjcD

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):

Database entries

  • Structure: 2PJR (product complex, complex with sulphate ion, Geobacillus stearothermophilus), 1PJR (substrate complex, complex with DNA, Geobacillus stearothermophilus)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Elisabeth Grohmann
Autonomous plasmid-like replication of Bacillus ICEBs1: a general feature of integrative conjugative elements?
Mol Microbiol: 2010, 75(2);261-3
[PubMed:19943906] [WorldCat.org] [DOI] (I p)

Original Publications

Additional publications: PubMed