PcrA

• Description: ATP-dependent DNA helicase, facilitates unwinding of ICEBs1 DNA for horizontal transfer
  
  

Gene name	pcrA
Synonyms	yerF
Essential	yes PubMed
Product	ATP-dependent DNA helicase
Function	plasmid rolling-circle replication, conjugative transfer of ICEBs1
Gene expression levels in SubtiExpress: pcrA
Interactions involving this protein in SubtInteract: PcrA
MW, pI	83 kDa, 5.767
Gene length, protein length	2217 bp, 739 aa
Immediate neighbours	pcrB, ligA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, mobile genetic elements, essential genes

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

• Locus tag: BSU06610

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • PcrA anchors itself to the template duplex using the 2B subdomain and reels in the lagging strand, extruding a single-stranded loop PubMed
  • dismantles RecA filaments PubMed

• Protein family: uvrD-like helicase C-terminal domain (according to Swiss-Prot)

• Paralogous protein(s): YjcD

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • unwinding of the double-stranded ICEBs1 DNA is stimulated by HelP PubMed
• Interactions:
  • PcRA interacts with the RNA polymerase PubMed
  • YxaL-PcrA PubMed
  • YerB-PcrA PubMed
  • YwhK-PcrA PubMed

• Localization:

Database entries

• Structure: 2PJR (product complex, complex with sulphate ion, Geobacillus stearothermophilus), 1PJR (substrate complex, complex with DNA, Geobacillus stearothermophilus)

• UniProt: O34580

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: pcrB-pcrA-ligA-yerH PubMed

• Expression browser: pcrA PubMed

• Sigma factor:

• Regulation:
  • induced by DNA damage (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Elisabeth Grohmann
Autonomous plasmid-like replication of Bacillus ICEBs1: a general feature of integrative conjugative elements?
Mol Microbiol: 2010, 75(2);261-3
[PubMed:19943906] [WorldCat.org] [DOI] (I p)

Original Publications

Additional publications: PubMed