Difference between revisions of "YabT"

From SubtiWiki
Jump to: navigation, search
Line 1: Line 1:
−
* '''Description:''' similar to [[protein kinases and phosphatases|Ser/Thr kinase]] <br/><br/>
+
* '''Description:''' [[protein kinases and phosphatases|Ser/Thr kinase]], controls DNA integrity during spore development <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
Line 10: Line 10:
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
+
|style="background:#ABCDEF;" align="center"| '''Product''' || [[protein kinases and phosphatases|Ser/Thr kinase]]
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
+
|style="background:#ABCDEF;" align="center"|'''Function''' || control of DNA integrity during spore development
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU00660 yabT]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU00660 yabT]
 +
|-
 +
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/YabT YabT]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 9.943   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 9.943   
Line 35: Line 37:
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
−
<br/><br/><br/><br/>
 
−
<br/><br/><br/><br/>
 
−
 
−
 
 
<br/><br/>
 
<br/><br/>
  
Line 55: Line 53:
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
* increased sensitivity to DNA damage during spore development {{PubMed|23634894}}
  
 
=== Database entries ===
 
=== Database entries ===
Line 71: Line 70:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
−
* '''Catalyzed reaction/ biological activity:''' ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)  
+
* '''Catalyzed reaction/ biological activity:'''  
 +
** ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)  
 +
** phosphorylation of [[RecA]] on Ser-2 {{PubMed|23634894}}
  
 
* '''Protein family:''' [[protein kinases and phosphatases|protein kinase]] domain (according to Swiss-Prot)
 
* '''Protein family:''' [[protein kinases and phosphatases|protein kinase]] domain (according to Swiss-Prot)
Line 88: Line 89:
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
 +
** autophosphorylation is stimulated by non-specific binding to DNA {{PubMed|23634894}}
  
 
* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
 +
** [[YabT]]-[[RecA]] {{PubMed|23634894}}
  
 
* '''[[Localization]]:'''
 
* '''[[Localization]]:'''
 +
** colocalizes strongly with the septal membrane separating the mother cells from the forespore {{PubMed|23634894}}
  
 
=== Database entries ===
 
=== Database entries ===
Line 111: Line 115:
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yabT_73809_74825_1 yabT] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yabT_73809_74825_1 yabT] {{PubMed|22383849}}
  
−
* '''Sigma factor:''' [[SigF]] {{PubMed|16497325}}
+
* '''[[Sigma factor]]:''' [[SigF]] {{PubMed|16497325}}
  
 
* '''Regulation:''' expressed early during sporulation in the forespore ([[SigF]]) {{PubMed|16497325}}
 
* '''Regulation:''' expressed early during sporulation in the forespore ([[SigF]]) {{PubMed|16497325}}
Line 121: Line 125:
 
=Biological materials =
 
=Biological materials =
  
−
* '''Mutant:''' GP577 (erm), available in [[Stülke]] lab  
+
* '''Mutant:''' GP577 (erm), available in [[Jörg Stülke]]'s lab  
  
 
* '''Expression vector:'''  
 
* '''Expression vector:'''  
−
** purification from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP391, available in [[Stülke]] lab  
+
** purification from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP391, available in [[Jörg Stülke]]'s lab  
−
** purification from ''E. coli'' with N-terminal Strep-tag, in [[pGP172]]: pGP823, available in [[Stülke]] lab  
+
** purification from ''E. coli'' with N-terminal Strep-tag, in [[pGP172]]: pGP823, available in [[Jörg Stülke]]'s lab  
−
** purification from ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP1408, available in [[Stülke]] lab  
+
** purification from ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP1408, available in [[Jörg Stülke]]'s lab  
  
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
−
* translational lacZ fusion (in [[pAC7]]): pGP831, available in [[Stülke]] lab  
+
* translational lacZ fusion (in [[pAC7]]): pGP831, available in [[Jörg Stülke]]'s lab  
  
 
* '''GFP fusion:'''
 
* '''GFP fusion:'''
Line 143: Line 147:
 
=References=
 
=References=
  
−
<pubmed>16497325,, </pubmed>
+
<pubmed>16497325,23634894, </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 18:39, 6 May 2013

  • Description: Ser/Thr kinase, controls DNA integrity during spore development

Gene name yabT
Synonyms
Essential no
Product Ser/Thr kinase
Function control of DNA integrity during spore development
Gene expression levels in SubtiExpress: yabT
Interactions involving this protein in SubtInteract: YabT
MW, pI 37 kDa, 9.943
Gene length, protein length 1014 bp, 338 aa
Immediate neighbours yabS, tilS
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YabT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YabT expression.png















Categories containing this gene/protein

protein modification, sporulation proteins

This gene is a member of the following regulons

SigF regulon

The gene

Basic information

  • Locus tag: BSU00660

Phenotypes of a mutant

  • increased sensitivity to DNA damage during spore development PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)
    • phosphorylation of RecA on Ser-2 PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • autophosphorylation is stimulated by non-specific binding to DNA PubMed
  • Localization:
    • colocalizes strongly with the septal membrane separating the mother cells from the forespore PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation: expressed early during sporulation in the forespore (SigF) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
    • purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP391, available in Jörg Stülke's lab
    • purification from E. coli with N-terminal Strep-tag, in pGP172: pGP823, available in Jörg Stülke's lab
    • purification from E. coli with N-terminal His-tag, in pWH844: pGP1408, available in Jörg Stülke's lab
  • lacZ fusion:
  • translational lacZ fusion (in pAC7): pGP831, available in Jörg Stülke's lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Vladimir Bidnenko, Lei Shi, Ahasanul Kobir, Magali Ventroux, Nathalie Pigeonneau, Céline Henry, Alain Trubuil, Marie-Françoise Noirot-Gros, Ivan Mijakovic
Bacillus subtilis serine/threonine protein kinase YabT is involved in spore development via phosphorylation of a bacterial recombinase.
Mol Microbiol: 2013, 88(5);921-35
[PubMed:23634894] [WorldCat.org] [DOI] (I p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Retrieved from "https://subtiwiki.uni-goettingen.de/wiki//index.php?title=YabT&oldid=179664"