Difference between revisions of "PutR"

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|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of proline utilization
 
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of proline utilization
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU03230 putR]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU03230 putR]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 47 kDa, 5.37   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 47 kDa, 5.37   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[putP]]'', ''[[ycgQ]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[putP]]'', ''[[ycgQ]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12117&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU03230 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU03230 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU03230 Advanced_DNA]
 
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Revision as of 12:06, 13 May 2013

  • Description: transcriptional activator of the proline utilization operon putB-putC-putP

Gene name putR
Synonyms ycgP, prcR
Essential no
Product transcriptional activator (PucR family)
Function regulation of proline utilization
Gene expression levels in SubtiExpress: putR
MW, pI 47 kDa, 5.37
Gene length, protein length 1233 bp, 411 aa
Immediate neighbours putP, ycgQ
Sequences Protein DNA Advanced_DNA
Genetic context
YcgP context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PutR expression.png


























Categories containing this gene/protein

transcription factors and their control, utilization of amino acids

This gene is a member of the following regulons

The PutR regulon:

The gene

Basic information

  • Locus tag: BSU03230

Phenotypes of a mutant

  • no growth with proline as single source of carbon or nitrogen PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: transcription activation of the proline utilization operon putB-putC-putP in the presence of proline PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): proline acts as co-activator PubMed
  • Effectors of protein activity: proline activates PutR PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: constitutively expressed PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Boris R Belitsky
Indirect repression by Bacillus subtilis CodY via displacement of the activator of the proline utilization operon.
J Mol Biol: 2011, 413(2);321-36
[PubMed:21840319] [WorldCat.org] [DOI] (I p)