Difference between revisions of "PrfA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || translation  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || translation  
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU37010 prfA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37010 prfA]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 4.824   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 4.824   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywkE]]'', ''[[ywkD]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywkE]]'', ''[[ywkD]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15718&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37010 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37010 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37010 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:prfA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:prfA_context.gif]]

Revision as of 14:05, 13 May 2013

  • Description: peptide chain release factor 1

Gene name prfA
Synonyms
Essential yes PubMed
Product peptide chain release factor 1
Function translation
Gene expression levels in SubtiExpress: prfA
MW, pI 40 kDa, 4.824
Gene length, protein length 1068 bp, 356 aa
Immediate neighbours ywkE, ywkD
Sequences Protein DNA Advanced_DNA
Genetic context
PrfA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PrfA expression.png
























Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU37010

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: binds to the ribosome through specific recognition of messenger RNA stop codons and triggers hydrolysis of the bond between the nascent polypeptide and the transfer RNA at the peptidyl-tRNA site, thereby releasing the newly synthesized protein. PrfA is highly specific for a U in the first stop-codon position and recognizes UAA and UAG
  • Protein family: prokaryotic/mitochondrial release factor family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Johan Sund, Martin Andér, Johan Aqvist
Principles of stop-codon reading on the ribosome.
Nature: 2010, 465(7300);947-50
[PubMed:20512119] [WorldCat.org] [DOI] (I p)

Daniel J Rigden, Peter Setlow, Barbara Setlow, Irina Bagyan, Richard A Stein, Mark J Jedrzejas
PrfA protein of Bacillus species: prediction and demonstration of endonuclease activity on DNA.
Protein Sci: 2002, 11(10);2370-81
[PubMed:12237459] [WorldCat.org] [DOI] (P p)