FliW

• Description: checkpoint protein for hag expression, CsrA anatagonist
  
  

Gene name	fliW
Synonyms	yviF
Essential	no
Product	CsrA anatagonist
Function	control of CsrA activity
Gene expression levels in SubtiExpress: fliW
Interactions involving this protein in SubtInteract: FliW
MW, pI	16 kDa, 4.338
Gene length, protein length	429 bp, 143 aa
Immediate neighbours	csrA, yviE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis

The gene

Basic information

• Locus tag: BSU35380

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • FliW binds CsrA when Hag is secreted after hook completion (and FliW released from the Hag-FliW complex) to allow translation of the hag mRNA PubMed

• Protein family: fliW family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • FliW-Hag PubMed
  • FliW-CsrA PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P96503

• KEGG entry: [2]

• E.C. number:

Additional information

• CsrA and FliW form a conserved module in many bacteria PubMed

Expression and regulation

• Operon:

• Expression browser: fliW PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Additional Reviews: PubMed

Original Publications

Sampriti Mukherjee, Paul Babitzke, Daniel B Kearns
FliW and FliS function independently to control cytoplasmic flagellin levels in Bacillus subtilis.
J Bacteriol: 2013, 195(2);297-306
[PubMed:23144244] [WorldCat.org] [DOI] (I p)

Sampriti Mukherjee, Helen Yakhnin, Dave Kysela, Josh Sokoloski, Paul Babitzke, Daniel B Kearns
CsrA-FliW interaction governs flagellin homeostasis and a checkpoint on flagellar morphogenesis in Bacillus subtilis.
Mol Microbiol: 2011, 82(2);447-61
[PubMed:21895793] [WorldCat.org] [DOI] (I p)

Björn Titz, Seesandra V Rajagopala, Claudia Ester, Roman Häuser, Peter Uetz
Novel conserved assembly factor of the bacterial flagellum.
J Bacteriol: 2006, 188(21);7700-6
[PubMed:16936039] [WorldCat.org] [DOI] (P p)