Difference between revisions of "LevB"

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|style="background:#ABCDEF;" align="center"|'''Function''' || levan degradation
 
|style="background:#ABCDEF;" align="center"|'''Function''' || levan degradation
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU34460 levB]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34460 levB]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sacB]]'', ''[[yveA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sacB]]'', ''[[yveA]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15451&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU34460 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU34460 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU34460 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:yveB_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yveB_context.gif]]

Revision as of 13:55, 13 May 2013

  • Description: endolevanase

Gene name levB
Synonyms yveB
Essential no
Product endolevanase
Function levan degradation
Gene expression levels in SubtiExpress: levB
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 58 kDa, 4.993
Gene length, protein length 1548 bp, 516 aa
Immediate neighbours sacB, yveA
Sequences Protein DNA Advanced_DNA
Genetic context
YveB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
LevB expression.png




























Categories containing this gene/protein

utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

DegU regulon, SacY regulon

The gene

Basic information

  • Locus tag: BSU34460

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Hydrolysis of (2->6)-beta-D-fructofuranan, to remove successive disaccharide residues as levanbiose, i (according to Swiss-Prot)
  • Protein family: glycosyl hydrolase 32 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induction by sucrose (at high concentration) (SacY) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Y Pereira, M F Petit-Glatron, R Chambert
yveB, Encoding endolevanase LevB, is part of the sacB-yveB-yveA levansucrase tricistronic operon in Bacillus subtilis.
Microbiology (Reading): 2001, 147(Pt 12);3413-9
[PubMed:11739774] [WorldCat.org] [DOI] (P p)

S Aymerich, M Steinmetz
Cloning and preliminary characterization of the sacS locus from Bacillus subtilis which controls the regulation of the exoenzyme levansucrase.
Mol Gen Genet: 1987, 208(1-2);114-20
[PubMed:3039303] [WorldCat.org] [DOI] (P p)

H Shimotsu, D J Henner
Modulation of Bacillus subtilis levansucrase gene expression by sucrose and regulation of the steady-state mRNA level by sacU and sacQ genes.
J Bacteriol: 1986, 168(1);380-8
[PubMed:2428811] [WorldCat.org] [DOI] (P p)