Difference between revisions of "McpB"

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|style="background:#ABCDEF;" align="center"|'''Function''' || control of chemotaxis
 
|style="background:#ABCDEF;" align="center"|'''Function''' || control of chemotaxis
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU31260 mcpB]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31260 mcpB]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/McpB McpB]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/McpB McpB]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tlpA]]'', ''[[tgl]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tlpA]]'', ''[[tgl]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15104&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31260 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31260 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31260 Advanced_DNA]
 
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Revision as of 13:42, 13 May 2013

  • Description: membrane-bound chemotaxis receptor, methyl-accepting chemotaxis protein, receptor for asparagine

Gene name mcpB
Synonyms
Essential no
Product methyl-accepting chemotaxis protein
Function control of chemotaxis
Gene expression levels in SubtiExpress: mcpB
Interactions involving this protein in SubtInteract: McpB
MW, pI 71 kDa, 5.13
Gene length, protein length 1986 bp, 662 aa
Immediate neighbours tlpA, tgl
Sequences Protein DNA Advanced_DNA
Genetic context
McpB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
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Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

  • Locus tag: BSU31260

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): McpA

Extended information on the protein

  • Kinetic information: K(D) for asparagine: 14 myM PubMed
  • Domains:
  • Modification: methylated at residues Gln371, Glu630, and Glu637 PubMed
  • Cofactor(s):
  • Effectors of protein activity: asparagine (binds to the part of McpB that is exposed to the exterior) PubMed
  • Localization:
    • cell membrane PubMed
    • localized at the cell poles, but in the presence of high asparagine concentration there is a reversible re-localization to the sides of the cell (lateral clusters) PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • in minimal medium, McpB is present with 6,200 +/- 1,900 molecules per cell PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References