Difference between revisions of "TlpA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || control of chemotaxis  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || control of chemotaxis  
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU31250 tlpA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31250 tlpA]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 72 kDa, 4.906   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 72 kDa, 4.906   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mcpA]]'', ''[[mcpB]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mcpA]]'', ''[[mcpB]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15103&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31250 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31250 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31250 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:tlpA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:tlpA_context.gif]]

Revision as of 13:42, 13 May 2013

  • Description: membrane-bound chemotaxis receptor, methyl-accepting chemotaxis protein

Gene name tlpA
Synonyms
Essential no
Product methyl-accepting chemotaxis protein
Function control of chemotaxis
Gene expression levels in SubtiExpress: tlpA
MW, pI 72 kDa, 4.906
Gene length, protein length 1986 bp, 662 aa
Immediate neighbours mcpA, mcpB
Sequences Protein DNA Advanced_DNA
Genetic context
TlpA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TlpA expression.png
























Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

AbrB regulon, SigD regulon

The gene

Basic information

  • Locus tag: BSU31250

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • in minimal medium, TlpA is present with 2,100 +/- 890 molecules per cell PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Vincent J Cannistraro, George D Glekas, Christopher V Rao, George W Ordal
Cellular stoichiometry of the chemotaxis proteins in Bacillus subtilis.
J Bacteriol: 2011, 193(13);3220-7
[PubMed:21515776] [WorldCat.org] [DOI] (I p)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

D W Hanlon, G W Ordal
Cloning and characterization of genes encoding methyl-accepting chemotaxis proteins in Bacillus subtilis.
J Biol Chem: 1994, 269(19);14038-46
[PubMed:8188684] [WorldCat.org] (P p)