Difference between revisions of "MetK"
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|style="background:#ABCDEF;" align="center"|'''Function''' || S-adenosylmethionine synthesis | |style="background:#ABCDEF;" align="center"|'''Function''' || S-adenosylmethionine synthesis | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU30550 metK] |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/cys_meth_and_sulfate_assimilation.html Cys, Met & Sulfate assimilation]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/cys_meth_and_sulfate_assimilation.html Cys, Met & Sulfate assimilation]''' | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[asnB]]'', ''[[pckA]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[asnB]]'', ''[[pckA]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU30550 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU30550 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU30550 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:metK_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:metK_context.gif]] | ||
Revision as of 13:40, 13 May 2013
- Description: S-adenosylmethionine synthetase
| Gene name | metK |
| Synonyms | metE |
| Essential | yes PubMed |
| Product | S-adenosylmethionine synthetase |
| Function | S-adenosylmethionine synthesis |
| Gene expression levels in SubtiExpress: metK | |
| Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation | |
| MW, pI | 43 kDa, 5.025 |
| Gene length, protein length | 1200 bp, 400 aa |
| Immediate neighbours | asnB, pckA |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
biosynthesis/ acquisition of amino acids, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU30550
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine (according to Swiss-Prot)
- Protein family: AdoMet synthetase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- UniProt: P54419
- KEGG entry: [3]
- E.C. number: 2.5.1.6
Additional information
Expression and regulation
- Sigma factor:
- Regulatory mechanism:
- S-box: riboswitch, binds S-adenosylmethionine resulting in transcription termination PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Jerneja Tomsic, Brooke A McDaniel, Frank J Grundy, Tina M Henkin
Natural variability in S-adenosylmethionine (SAM)-dependent riboswitches: S-box elements in bacillus subtilis exhibit differential sensitivity to SAM In vivo and in vitro.
J Bacteriol: 2008, 190(3);823-33
[PubMed:18039762]
[WorldCat.org]
[DOI]
(I p)
Brooke A McDaniel, Frank J Grundy, Vineeta P Kurlekar, Jerneja Tomsic, Tina M Henkin
Identification of a mutation in the Bacillus subtilis S-adenosylmethionine synthetase gene that results in derepression of S-box gene expression.
J Bacteriol: 2006, 188(10);3674-81
[PubMed:16672621]
[WorldCat.org]
[DOI]
(P p)
F J Grundy, T M Henkin
The S box regulon: a new global transcription termination control system for methionine and cysteine biosynthesis genes in gram-positive bacteria.
Mol Microbiol: 1998, 30(4);737-49
[PubMed:10094622]
[WorldCat.org]
[DOI]
(P p)
Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221]
[WorldCat.org]
[DOI]
(P p)
