Difference between revisions of "MutM"

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|style="background:#ABCDEF;" align="center"|'''Function''' || DNA repair  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || DNA repair  
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU29080 mutM]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29080 mutM]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 8.889   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 8.889   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ytaF]]'', ''[[polA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ytaF]]'', ''[[polA]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14868&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29080 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29080 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29080 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:mutM_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:mutM_context.gif]]

Revision as of 13:34, 13 May 2013

  • Description: formamidopyrimidine-DNA glycosidase

Gene name mutM
Synonyms ytaE
Essential no
Product formamidopyrimidine-DNA glycosidase
Function DNA repair
Gene expression levels in SubtiExpress: mutM
MW, pI 31 kDa, 8.889
Gene length, protein length 834 bp, 278 aa
Immediate neighbours ytaF, polA
Sequences Protein DNA Advanced_DNA
Genetic context
MutM context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MutM expression.png
























Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU29080

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine (according to Swiss-Prot)
  • Protein family: FPG family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2F5S (complex with oxoG:C, Geobacillus stearothermophilus)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Yan Qi, Marie C Spong, Kwangho Nam, Martin Karplus, Gregory L Verdine
Entrapment and structure of an extrahelical guanine attempting to enter the active site of a bacterial DNA glycosylase, MutM.
J Biol Chem: 2010, 285(2);1468-78
[PubMed:19889642] [WorldCat.org] [DOI] (I p)

Luz E Vidales, Lluvia C Cárdenas, Eduardo Robleto, Ronald E Yasbin, Mario Pedraza-Reyes
Defects in the error prevention oxidized guanine system potentiate stationary-phase mutagenesis in Bacillus subtilis.
J Bacteriol: 2009, 191(2);506-13
[PubMed:19011023] [WorldCat.org] [DOI] (I p)