SdhA

• Description: succinate dehydrogenase (flavoprotein subunit)
  
  

Gene name	sdhA
Synonyms	citF
Essential	no
Product	succinate dehydrogenase (flavoprotein subunit)
Function	TCA cycle
Gene expression levels in SubtiExpress: sdhA
Interactions involving this protein in SubtInteract: SdhA
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	65 kDa, 5.714
Gene length, protein length	1758 bp, 586 aa
Immediate neighbours	sdhB, sdhC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, membrane proteins, phosphoproteins

This gene is a member of the following regulons

FsrA regulon

The gene

Basic information

• Locus tag: BSU28440

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Succinate + acceptor = fumarate + reduced acceptor (according to Swiss-Prot)

• Protein family: FRD/SDH subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated PubMed, PubMed

• Cofactor(s): Fe

• Effectors of protein activity:
  • Inhibited by 2-(n-Heptyl)-4-hydroxy-quinoline N-oxide PubMed
  • Activated by Cytochrome b558 PubMed

• Interactions:
  • SdhA-SdhB (as part of the SdhC-SdhB-SdhA complex)
  • FloT-SdhA PubMed

• Localization: attached to the membrane PubMed

Database entries

• Structure: 1NEK (E. coli)

• UniProt: P08065

• KEGG entry: [3]

• E.C. number: 1.3.99.1

Additional information

• This enzyme is a membrane-bound trimer PubMed PubMed
  • One subunit is bound to cytochrome b558, and this subunit is the one bound to the cytosolic side of the membrane PubMed PubMed
  • Another subunit is the flavoprotein one, required for FAD usage PubMed PubMed
  • The other subunit has an iron-sulphur domain necessary for the catalytic activity PubMed PubMed
• extensive information on the structure and enzymatic properties of succinate dehydrogenase can be found at Proteopedia

Expression and regulation

• Operon: sdhC-sdhA-sdhB PubMed

• Expression browser: sdhA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • constitutive
  • part of the iron sparing response (FsrA) PubMed

• Regulatory mechanism:
  • FsrA: translation inhibition PubMed

• Additional information:

Biological materials

• Mutant: GP743 (sdhCA, cat), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody: