MccA

• Description: O-acetylserine-thiol-lyase
  
  

Gene name	mccA
Synonyms	yrhA
Essential	no
Product	O-acetylserine-thiol-lyase
Function	methionine-to-cysteine conversion
Gene expression levels in SubtiExpress: mccA
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	32 kDa, 5.227
Gene length, protein length	921 bp, 307 aa
Immediate neighbours	mccB, mtnN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

CymR regulon, Spx regulon

The gene

Basic information

• Locus tag: BSU27260

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: O(3)-acetyl-L-serine + L-homocysteine = L-cystathionine + acetate (according to Swiss-Prot)

• Protein family: cysteine synthase/cystathionine beta-synthase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O05393

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yrrT-mtnN-mccA-mccB-yrhC PubMed

• Expression browser: mccA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of cysteine (CymR) PubMed

• Regulatory mechanism:
  • CymR: transcription repression PubMed
  • Spx: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A945 ( mccA::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Marie-Françoise Hullo, Sandrine Auger, Olga Soutourina, Octavian Barzu, Mireille Yvon, Antoine Danchin, Isabelle Martin-Verstraete
Conversion of methionine to cysteine in Bacillus subtilis and its regulation.
J Bacteriol: 2007, 189(1);187-97
[PubMed:17056751] [WorldCat.org] [DOI] (P p)

Soon-Yong Choi, Dindo Reyes, Montira Leelakriangsak, Peter Zuber
The global regulator Spx functions in the control of organosulfur metabolism in Bacillus subtilis.
J Bacteriol: 2006, 188(16);5741-51
[PubMed:16885442] [WorldCat.org] [DOI] (P p)

Sergine Even, Pierre Burguière, Sandrine Auger, Olga Soutourina, Antoine Danchin, Isabelle Martin-Verstraete
Global control of cysteine metabolism by CymR in Bacillus subtilis.
J Bacteriol: 2006, 188(6);2184-97
[PubMed:16513748] [WorldCat.org] [DOI] (P p)

Shunji Nakano, Michiko M Nakano, Ying Zhang, Montira Leelakriangsak, Peter Zuber
A regulatory protein that interferes with activator-stimulated transcription in bacteria.
Proc Natl Acad Sci U S A: 2003, 100(7);4233-8
[PubMed:12642660] [WorldCat.org] [DOI] (P p)