Difference between revisions of "CwlA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || minor autolysin
 
|style="background:#ABCDEF;" align="center"|'''Function''' || minor autolysin
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU25900 cwlA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25900 cwlA]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 10.4   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 10.4   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqxI]]'', ''[[yqxH]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqxI]]'', ''[[yqxH]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14531&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25900 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25900 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25900 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:cwlA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:cwlA_context.gif]]

Revision as of 13:22, 13 May 2013

  • Description: N-acetylmuramoyl-L-alanine amidase

Gene name cwlA
Synonyms
Essential no
Product N-acetylmuramoyl-L-alanine amidase
Function minor autolysin
Gene expression levels in SubtiExpress: cwlA
MW, pI 29 kDa, 10.4
Gene length, protein length 816 bp, 272 aa
Immediate neighbours yqxI, yqxH
Sequences Protein DNA Advanced_DNA
Genetic context
CwlA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CwlA expression.png
























Categories containing this gene/protein

cell wall degradation/ turnover, Skin element

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU25900

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)
  • Protein family: N-acetylmuramoyl-L-alanine amidase 2 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

A Kuroda, M Imazeki, J Sekiguchi
Purification and characterization of a cell wall hydrolase encoded by the cwlA gene of Bacillus subtilis.
FEMS Microbiol Lett: 1991, 65(1);9-13
[PubMed:1908396] [WorldCat.org] [DOI] (P p)