Difference between revisions of "MifM"
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU23880 mifM] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU23880 mifM] | ||
| + | |- | ||
| + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/MifM MifM] | ||
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 11 kDa, 5.829 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 11 kDa, 5.829 | ||
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* '''Domains:''' | * '''Domains:''' | ||
| + | ** N-terminal transmembrane domain {{PubMed|22864117}} | ||
| + | ** the C-terminal domain interacts with the ribosome and arrests the own translation elongation {{PubMed|22864117}} | ||
* '''Modification:''' | * '''Modification:''' | ||
| Line 85: | Line 89: | ||
* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
** the C-terminus of MifM interacts with the ribosomal polypeptide exit tunnel {{PubMed|19779460}} | ** the C-terminus of MifM interacts with the ribosomal polypeptide exit tunnel {{PubMed|19779460}} | ||
| + | ** [[SpoIIIJ]]-[[MifM]] {{PubMed|22864117}} | ||
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
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* '''Additional information:''' | * '''Additional information:''' | ||
** An [[ncRNA|antisense RNA]] is predicted for ''[[mifM]]'' {{PubMed|20525796}} | ** An [[ncRNA|antisense RNA]] is predicted for ''[[mifM]]'' {{PubMed|20525796}} | ||
| − | * | + | ** MifM has intrinsic sequences to arrest ribosome function, the arrest is influenced by the cotranslational engagement in the protein localization reaction {{PubMed|22864117}} |
| + | ** elongation proceeds when [[SpoIIIJ]] integrates the nascent chain of [[MifM]] into the membrane {{PubMed|22864117}} | ||
=Biological materials = | =Biological materials = | ||
Revision as of 13:03, 7 August 2012
- Description: ribosome-nascent chain sensor of membrane protein biogenesis
| Gene name | mifM |
| Synonyms | yqzJ |
| Essential | no |
| Product | sensor of SpoIIIJ activity |
| Function | control of yidC2 translation |
| Gene expression levels in SubtiExpress: mifM | |
| Interactions involving this protein in SubtInteract: MifM | |
| MW, pI | 11 kDa, 5.829 |
| Gene length, protein length | 285 bp, 95 aa |
| Immediate neighbours | polY1, yidC2 |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
translation, membrane proteins
This gene is a member of the following regulons
The MifM regulon: yidC2
The gene
Basic information
- Locus tag: BSU23880
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: acts as a kind of "leader peptide" that does or does not allow translation through a hairpin structure in the yidC2 mRNA PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- membrane PubMed
Database entries
- Structure:
- UniProt: Q7WY64
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
- An antisense RNA is predicted for mifM PubMed
- MifM has intrinsic sequences to arrest ribosome function, the arrest is influenced by the cotranslational engagement in the protein localization reaction PubMed
- elongation proceeds when SpoIIIJ integrates the nascent chain of MifM into the membrane PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
