Difference between revisions of "SerA"

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* '''Modification:'''  
 
* '''Modification:'''  
 
** S-cysteinylation after diamide stress (C410)[http://www.ncbi.nlm.nih.gov/sites/entrez/17611193 PubMed]
 
** S-cysteinylation after diamide stress (C410)[http://www.ncbi.nlm.nih.gov/sites/entrez/17611193 PubMed]
** S-bacillithiolation upon hypochlorite stress on Cys-410 {{PubMed|21749987}}  
+
** active site Cys410 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species {{PubMed|21749987}} {{PubMed|22938038}}
  
 
* '''Cofactor(s):'''
 
* '''Cofactor(s):'''

Revision as of 08:45, 6 September 2012

  • Description: phosphoglycerate dehydrogenase

Gene name serA
Synonyms
Essential no
Product phosphoglycerate dehydrogenase
Function biosynthesis of serine
Gene expression levels in SubtiExpress: serA
Metabolic function and regulation of this protein in SubtiPathways:
Ala, Gly, Ser
MW, pI 56 kDa, 5.617
Gene length, protein length 1575 bp, 525 aa
Immediate neighbours ypzE, aroC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SerA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SerA expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU23070

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 3-phospho-D-glycerate + NAD+ = 3-phosphonooxypyruvate + NADH (according to Swiss-Prot)
  • Protein family: D-isomer specific 2-hydroxyacid dehydrogenase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • S-cysteinylation after diamide stress (C410)PubMed
    • active site Cys410 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1YBA (from E. coli, 30% identity, 52% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion: pGP187 (in pAC7), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References