Difference between revisions of "PrpC"

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* '''Mutant:''' OMG401 (aphA3), available in the [[Stülke]] lab
 
* '''Mutant:''' OMG401 (aphA3), available in the [[Stülke]] lab
 +
** 1A962 (no resistance), {{PubMed|12399479}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A962&Search=1A962 BGSC]
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 10:44, 20 September 2012

Gene name prpC
Synonyms yloO
Essential no
Product protein phosphatase
Function antagonist of PrkC-dependent phosphorylation
Gene expression levels in SubtiExpress: prpC
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 27 kDa, 4.355
Gene length, protein length 762 bp, 254 aa
Immediate neighbours yloN, prkC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PrpC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PrpC expression.png




























Categories containing this gene/protein

protein modification

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU15760

Phenotypes of a mutant

A prpC mutant is less lytic in late stationary phase. PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: A phosphoprotein + H2O = a protein + phosphate (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Proteins dephosphorylated by PrpC

CpgA, EF-Tu, YezB PubMed, HPr PubMed

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): divalent cations such as magnesium or manganese
  • Effectors of protein activity: inhibited by inorganic phosphate and glycero-2-phosphate PubMed

Database entries

  • Structure: 1TXO (from Mycobacterium tuberculosis, 34% identity, 54% similarity) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: OMG401 (aphA3), available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications

Additional publications: PubMed