Difference between revisions of "SepF"
Line 36: | Line 36: | ||
__TOC__ | __TOC__ | ||
− | |||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
− | |||
− | |||
− | |||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
Line 94: | Line 90: | ||
* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
− | ** [[ | + | ** [[FtsZ]] (extreme C terminus of [[FtsZ]])-[[SepF]] {{PubMed|22912848,16420366}} |
* '''[[Localization]]:''' septum {{PubMed|16420366}} | * '''[[Localization]]:''' septum {{PubMed|16420366}} | ||
Line 155: | Line 151: | ||
==Original Publications== | ==Original Publications== | ||
'''Additional publications:''' {{PubMed|21224850,22122227}} | '''Additional publications:''' {{PubMed|21224850,22122227}} | ||
− | <pubmed>16420366,16796675,14651647, </pubmed> | + | <pubmed>16420366,16796675,14651647, 22912848</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 11:53, 23 August 2012
- Description: part of the divisome
Gene name | sepF |
Synonyms | ylmF |
Essential | no |
Product | FtsZ-interacting protein |
Function | proper execution of septum synthesis |
Gene expression levels in SubtiExpress: sepF | |
Interactions involving this protein in SubtInteract: SepF | |
MW, pI | 17 kDa, 4.863 |
Gene length, protein length | 447 bp, 149 aa |
Immediate neighbours | ylmE, ylmG |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU15390
Phenotypes of a mutant
- perturbation of the formation of properly formed division septa
- less efficient cell division results in longer cells. Electron microscopy reveals strongly distorted division septa.
- the sepF mutation in combination with a constitutively active form of WalR (WalR-R204C) results in the formation of cell wall-less L-forms PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: sepF family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: septum PubMed
Database entries
- Structure:
- UniProt: O31728
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Leendert Hamoen, CBCB, Newcastle University, UK
Shu Ishikawa, Nara Institute of Science and Technology, Nara, Japan
Your additional remarks
SepF mutation is synthetic lethal in combination with an ezrA mutation or an ftsA mutation.
References
Reviews
Kevin M Devine
Bacterial L-forms on tap: an improved methodology to generate Bacillus subtilis L-forms heralds a new era of research.
Mol Microbiol: 2012, 83(1);10-3
[PubMed:22126136]
[WorldCat.org]
[DOI]
(I p)
David W Adams, Jeff Errington
Bacterial cell division: assembly, maintenance and disassembly of the Z ring.
Nat Rev Microbiol: 2009, 7(9);642-53
[PubMed:19680248]
[WorldCat.org]
[DOI]
(I p)
Original Publications
Additional publications: PubMed