Difference between revisions of "QueE"
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| − | * '''Description:''' synthesis of the modified ribonucleotide queuosine <br/><br/> | + | * '''Description:''' 7-carboxy-7-deazaguanine (CDG) synthase, required for the synthesis of the modified ribonucleotide queuosine <br/><br/> |
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
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| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || 7-carboxy-7-deazaguanine (CDG) synthase |
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || tRNA modification | |style="background:#ABCDEF;" align="center"|'''Function''' || tRNA modification | ||
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<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
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<br/><br/> | <br/><br/> | ||
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=== Additional information=== | === Additional information=== | ||
| − | |||
| − | |||
| − | |||
=The protein= | =The protein= | ||
=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| + | ** conversion of 6-carboxy-5,6,7,8-tetrahydropterin to 7-carboxy-7-deazaguanine (CDG) {{PubMed|23194065}} | ||
* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
| − | * '''Protein family:''' | + | * '''Protein family:''' radical S-adenosyl-L-methionine (SAM) superfamily |
* '''Paralogous protein(s):''' | * '''Paralogous protein(s):''' | ||
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* '''Modification:''' | * '''Modification:''' | ||
| − | * '''Cofactor(s):''' | + | * '''Cofactor(s):''' [4Fe-4S] cluster, S-adenosyl-L-methionine, Mg(2+) {{PubMed|23194065}} |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
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=References= | =References= | ||
| − | <pubmed>14660578,19285444, 19354300 17384645 </pubmed> | + | <pubmed>14660578,19285444, 19354300 17384645 23194065 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 15:51, 2 December 2012
- Description: 7-carboxy-7-deazaguanine (CDG) synthase, required for the synthesis of the modified ribonucleotide queuosine
| Gene name | queE |
| Synonyms | ykvL |
| Essential | no |
| Product | 7-carboxy-7-deazaguanine (CDG) synthase |
| Function | tRNA modification |
| Gene expression levels in SubtiExpress: queE | |
| MW, pI | 26 kDa, 4.962 |
| Gene length, protein length | 729 bp, 243 aa |
| Immediate neighbours | queD, queF |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU13740
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- conversion of 6-carboxy-5,6,7,8-tetrahydropterin to 7-carboxy-7-deazaguanine (CDG) PubMed
- Catalyzed reaction/ biological activity:
- Protein family: radical S-adenosyl-L-methionine (SAM) superfamily
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s): [4Fe-4S] cluster, S-adenosyl-L-methionine, Mg(2+) PubMed
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: O31677
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- repressed in the presence of queuosine (preQ1 riboswitch) PubMed
- Regulatory mechanism:
- preQ1 riboswitch: transcriptional antitermination in the absence of queuosine PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
