YqiI

• Description: secreted N-acetylmuramoyl-L-alanine amidase
  
  

Gene name	yqiI
Synonyms
Essential	no
Product	peptidoglycan hydrolase
Function	cell wall metabolism
Gene expression levels in SubtiExpress: yqiI
MW, pI	22 kDa, 4.286
Gene length, protein length	618 bp, 206 aa
Immediate neighbours	yqiK, yqiH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover

The gene

Basic information

• Locus tag: BSU24190

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • cell wall hydrolysis PubMed

• Protein family: N-acetylmuramoyl-L-alanine amidase 3 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: contains an amidase_3 domain (like CwlC, CwlD, LytC, YrvJ)

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • secreted (with N-terminal signal sequence for SEC-mediated transport) PubMed

Database entries

• Structure:

• UniProt: P54525

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yqiH-yqiI-yqiK PubMed

• Expression browser: yqiI PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • only expressed under conditions of high osmolarity PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Kathleen E Fischer, Erhard Bremer
Activity of the osmotically regulated yqiHIK promoter from Bacillus subtilis is controlled at a distance.
J Bacteriol: 2012, 194(19);5197-208
[PubMed:22843846] [WorldCat.org] [DOI] (I p)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)