Difference between revisions of "YerD"
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=Biological materials = | =Biological materials = | ||
| − | * '''Mutant:''' GP1184 (tet) available in [[Stülke]] lab | + | * '''Mutant:''' GP1184 (tet) available in [[Jörg Stülke]]'s lab |
* '''Expression vector:''' pBP32 (expression vector for ''E. coli'' BL21; N-terminal Strep-tag-YerD fusion protein, based on [[pGP172]]), available in [[Fabian Commichau]]'s lab | * '''Expression vector:''' pBP32 (expression vector for ''E. coli'' BL21; N-terminal Strep-tag-YerD fusion protein, based on [[pGP172]]), available in [[Fabian Commichau]]'s lab | ||
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* '''two-hybrid system:''' | * '''two-hybrid system:''' | ||
| − | * '''FLAG-tag construct:''' GP1187 (spc, based on [[pGP1331]]), available in [[Stülke]] lab | + | * '''FLAG-tag construct:''' GP1187 (spc, based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab |
* '''Antibody:''' | * '''Antibody:''' | ||
Revision as of 16:51, 17 July 2012
- Description: general stress protein, similar to glutamate synthase (ferredoxin)
| Gene name | yerD |
| Synonyms | |
| Essential | no |
| Product | unknown |
| Function | unknown |
| MW, pI | 58 kDa, 7.349 |
| Gene length, protein length | 1575 bp, 525 aa |
| Immediate neighbours | yerC, pcrB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
general stress proteins (controlled by SigB), poorly characterized/ putative enzymes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU06590
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s): GltA
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: O34849
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP1184 (tet) available in Jörg Stülke's lab
- Expression vector: pBP32 (expression vector for E. coli BL21; N-terminal Strep-tag-YerD fusion protein, based on pGP172), available in Fabian Commichau's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- FLAG-tag construct: GP1187 (spc, based on pGP1331), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
