Difference between revisions of "DnaD"
| Line 70: | Line 70: | ||
* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
** [[DnaD]]-mediated untwisting of the DNA stimulates [[Nth]] endonuclease activity at AP sites {{PubMed|21954439}} | ** [[DnaD]]-mediated untwisting of the DNA stimulates [[Nth]] endonuclease activity at AP sites {{PubMed|21954439}} | ||
| + | ** required for the assembly of the replicative helicase DnaC at the origin of replication {{PubMed|22821970}} | ||
| + | ** interaction with DnaA inhibits the ability of DnaA to bind cooperatively to DNA {{PubMed|22821970}} | ||
* '''Protein family:''' DNA polymerase type-Y family (according to Swiss-Prot) | * '''Protein family:''' DNA polymerase type-Y family (according to Swiss-Prot) | ||
| Line 89: | Line 91: | ||
* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
** [[DnaD]]-[[PriA]] {{PubMed|11585815}} | ** [[DnaD]]-[[PriA]] {{PubMed|11585815}} | ||
| − | ** [[DnaD]]-[[DnaA]] {{PubMed|11222620}} | + | ** [[DnaD]]-[[DnaA]] {{PubMed|22821970,11222620}} |
** [[DnaD]]-[[DnaB]] {{PubMed|15186423,19707554}} | ** [[DnaD]]-[[DnaB]] {{PubMed|15186423,19707554}} | ||
** part of the [[replisome]]: [[PolC]]-[[HolA]]-[[HolB]]-[[DnaX]]-[[DnaN]]-[[DnaG]]-[[DnaC]]-[[DnaI]]-[[DnaD]]-[[SsbA]]-[[DnaE]]-[[PriA]]-[[DnaB]] {{PubMed|20122408}} | ** part of the [[replisome]]: [[PolC]]-[[HolA]]-[[HolB]]-[[DnaX]]-[[DnaN]]-[[DnaG]]-[[DnaC]]-[[DnaI]]-[[DnaD]]-[[SsbA]]-[[DnaE]]-[[PriA]]-[[DnaB]] {{PubMed|20122408}} | ||
| Line 146: | Line 148: | ||
<pubmed> 22797751</pubmed> | <pubmed> 22797751</pubmed> | ||
==Original publications== | ==Original publications== | ||
| − | <pubmed> 19707554,15686560,15186423,11585815, 20122408,16479537, 11222620 19968790 11679082 20587500 21097613 21954439</pubmed> | + | <pubmed> 19707554,15686560,15186423,11585815, 20122408,16479537, 11222620 19968790 11679082 20587500 21097613 21954439 22821970</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 10:40, 24 July 2012
- Description: initiation of chromosome replication, part of the replisome
| Gene name | dnaD |
| Synonyms | |
| Essential | yes PubMed |
| Product | initiation of chromosome replication |
| Function | DNA replication |
| Interactions involving this protein in SubtInteract: DnaD | |
| MW, pI | 27 kDa, 7.174 |
| Gene length, protein length | 696 bp, 232 aa |
| Immediate neighbours | nth, asnS |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
DNA replication, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU22350
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: DNA polymerase type-Y family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: Membrane-proximal (Spotty) PubMed
Database entries
- Structure: 2C79 (N-terminal domain)
- UniProt: P39787
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
Operon: dnaD-nth PubMed
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Geoffrey S Briggs, Wiep Klaas Smits, Panos Soultanas
Chromosomal replication initiation machinery of low-G+C-content Firmicutes.
J Bacteriol: 2012, 194(19);5162-70
[PubMed:22797751]
[WorldCat.org]
[DOI]
(I p)
Original publications
