Difference between revisions of "YerD"

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(Biological materials)
(Biological materials)
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* '''Mutant:''' GP1184 (tet) available in [[Stülke]] lab
 
* '''Mutant:''' GP1184 (tet) available in [[Stülke]] lab
  
* '''Expression vector:''' pBP32 (N-terminal Strep-tag), available in [[Fabian Commichau]]'s lab
+
* '''Expression vector:''' pBP32 (expression vector for ''E. coli'' BL21; N-terminal Strep-tag-YerD fusion protein), available in [[Fabian Commichau]]'s lab
 
          
 
          
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''

Revision as of 16:19, 17 July 2012

  • Description: general stress protein, similar to glutamate synthase (ferredoxin)

Gene name yerD
Synonyms
Essential no
Product unknown
Function unknown
MW, pI 58 kDa, 7.349
Gene length, protein length 1575 bp, 525 aa
Immediate neighbours yerC, pcrB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YerD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YerD expression.png
























Categories containing this gene/protein

general stress proteins (controlled by SigB), poorly characterized/ putative enzymes

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

  • Locus tag: BSU06590

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): GltA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1184 (tet) available in Stülke lab
  • Expression vector: pBP32 (expression vector for E. coli BL21; N-terminal Strep-tag-YerD fusion protein), available in Fabian Commichau's lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • FLAG-tag construct: GP1187 (spc, based on pGP1331), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References