CheD

• Description: required for methylation of methyl-accepting chemotaxis proteins by CheR, enhances phosphatase activity of CheC
  
  

Gene name	cheD
Synonyms	ylxK
Essential	no
Product	CheC activity modulator
Function	motility and chemotaxis
Gene expression levels in SubtiExpress: cheD
Interactions involving this protein in SubtInteract: CheD
MW, pI	17 kDa, 9.347
Gene length, protein length	498 bp, 166 aa
Immediate neighbours	cheC, sigD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis

This gene is a member of the following regulons

CodY regulon, SigD regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU16460

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein L-glutamine + H₂O = protein L-glutamate + NH₃ (according to Swiss-Prot)

• Protein family: cheD family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • CheC-CheD PubMed

• Localization:

Database entries

• Structure:

• UniProt: P40404

• KEGG entry: [3]

• E.C. number: 3.5.1.44

Expression and regulation

• Operon:
  • flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-ylxH-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
  • ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-ylxH-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed

• Expression browser: cheD PubMed

• Sigma factor:
  • flgB: SigA PubMed, SigD PubMed
  • ylxF: SigD PubMed

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed
  • CodY: transcription repression PubMed
  • DegU (non-phosphorylated): transcription activation PubMed

• Additional information:
  • in minimal medium, CheD is present with 1,200 +/- 250 molecules per cell PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: