GanA

• Description: beta-galactosidase
  
  

Gene name	lacA
Synonyms	yvfN
Essential	no
Product	beta-galactosidase
Function	galactan utilization
Gene expression levels in SubtiExpress: lacA
MW, pI	79 kDa, 5.704
Gene length, protein length	2061 bp, 687 aa
Immediate neighbours	yvfO, yvfM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, phosphoproteins

This gene is a member of the following regulons

LacR regulon

The gene

Basic information

• Locus tag: BSU34130

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 42 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-387 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O07012

• KEGG entry: [3]

• E.C. number: 3.2.1.23 3

Expression and regulation

• Operon: cycB-yvfL-yvfM-lacA-yvfO PubMed

• Expression browser: lacA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • LacR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:GP1181 (aphA3) available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: