CydB

• Description: cytochrome bd ubiquinol oxidase (subunit II), high affinity terminal oxidase
  
  

Gene name	cydB
Synonyms	yxkL
Essential	no
Product	cytochrome bd ubiquinol oxidase (subunit II), high affinity terminal oxidase
Function	respiration
Gene expression levels in SubtiExpress: cydB
MW, pI	37 kDa, 9.48
Gene length, protein length	1014 bp, 338 aa
Immediate neighbours	cydC, cydA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

respiration, membrane proteins

This gene is a member of the following regulons

CcpA regulon, ResD regulon, Rex regulon

The gene

Basic information

• Locus tag: BSU38750

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Ubiquinol-8 + O₂ = Ubiquinone-8 + H₂O (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): contains an iron-sulfur cluster

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P94365

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cydA-cydB-cydC-cydD PubMed

• Expression browser: cydB PubMed

• Sigma factor:

• Regulation:
  • repressed by glucose (CcpA)
  • induced under anaerobic conditions (Rex) PubMed
  • expressed under anaerobic conditions (ResD) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression
  • Rex: transcription repression PubMed
  • ResD: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ankita Puri-Taneja, Matthew Schau, Yinghua Chen, F Marion Hulett
Regulators of the Bacillus subtilis cydABCD operon: identification of a negative regulator, CcpA, and a positive regulator, ResD.
J Bacteriol: 2007, 189(9);3348-58
[PubMed:17322317] [WorldCat.org] [DOI] (P p)

Jonas T Larsson, Annika Rogstam, Claes von Wachenfeldt
Coordinated patterns of cytochrome bd and lactate dehydrogenase expression in Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 10);3323-3335
[PubMed:16207915] [WorldCat.org] [DOI] (P p)

Matthew Schau, Yinghua Chen, F Marion Hulett
Bacillus subtilis YdiH is a direct negative regulator of the cydABCD operon.
J Bacteriol: 2004, 186(14);4585-95
[PubMed:15231791] [WorldCat.org] [DOI] (P p)

N Azarkina, S Siletsky, V Borisov, C von Wachenfeldt, L Hederstedt, A A Konstantinov
A cytochrome bb'-type quinol oxidase in Bacillus subtilis strain 168.
J Biol Chem: 1999, 274(46);32810-7
[PubMed:10551842] [WorldCat.org] [DOI] (P p)

L Winstedt, K Yoshida, Y Fujita, C von Wachenfeldt
Cytochrome bd biosynthesis in Bacillus subtilis: characterization of the cydABCD operon.
J Bacteriol: 1998, 180(24);6571-80
[PubMed:9852001] [WorldCat.org] [DOI] (P p)