NagB

• Description: glucosamine-6-phosphate deaminase
  
  

Gene name	nagB
Synonyms
Essential	no
Product	glucosamine-6-phosphate deaminase
Function	N-acetylglucosamine utilization
Gene expression levels in SubtiExpress: nagB
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism, Murein recycling
MW, pI	26 kDa, 5.717
Gene length, protein length	726 bp, 242 aa
Immediate neighbours	nagA, nagR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover, utilization of specific carbon sources

This gene is a member of the following regulons

NagR regulon

The gene

Basic information

• Locus tag: BSU35020

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-glucosamine 6-phosphate + H₂O = D-fructose 6-phosphate + NH₃ (according to Swiss-Prot)

• Protein family: NagB subfamily (according to Swiss-Prot)

• Paralogous protein(s): GamA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2BKXPubMed

• UniProt: O35000

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: nagA-nagB-nagR PubMed

• Expression browser: nagB PubMed

• Sigma factor:

• Regulation:
  • induced in the presence of N-acetylglucosamine (NagR) PubMed

• Regulatory mechanism:
  • NagR: transcription repression PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Christopher T Brown, Laura K Fishwick, Binna M Chokshi, Marissa A Cuff, Jay M Jackson, Travis Oglesby, Alison T Rioux, Enrique Rodriguez, Gregory S Stupp, Austin H Trupp, James S Woollcombe-Clarke, Tracy N Wright, William J Zaragoza, Jennifer C Drew, Eric W Triplett, Wayne L Nicholson
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Appl Environ Microbiol: 2011, 77(19);6867-77
[PubMed:21821766] [WorldCat.org] [DOI] (I p)

Florence Vincent, Gideon J Davies, James A Brannigan
Structure and kinetics of a monomeric glucosamine 6-phosphate deaminase: missing link of the NagB superfamily?
J Biol Chem: 2005, 280(20);19649-55
[PubMed:15755726] [WorldCat.org] [DOI] (P p)

H L Mobley, R J Doyle, U N Streips, S O Langemeier
Transport and incorporation of N-acetyl-D-glucosamine in Bacillus subtilis.
J Bacteriol: 1982, 150(1);8-15
[PubMed:6174502] [WorldCat.org] [DOI] (P p)