Difference between revisions of "PnbA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || lipid degradation  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || lipid degradation  
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU34390 pnbA]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 53 kDa, 4.776   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 53 kDa, 4.776   

Revision as of 16:14, 7 August 2012

  • Description: para-nitrobenzyl esterase

Gene name pnbA
Synonyms estB
Essential no
Product para-nitrobenzyl esterase
Function lipid degradation
Gene expression levels in SubtiExpress: pnbA
MW, pI 53 kDa, 4.776
Gene length, protein length 1467 bp, 489 aa
Immediate neighbours slrR, padC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PnbA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PnbA expression.png
























Categories containing this gene/protein

utilization of lipids, phosphoproteins

This gene is a member of the following regulons

AbrB regulon

The gene

Basic information

  • Locus tag: BSU34390

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Triacylglycerol + H2O = diacylglycerol + a carboxylate (according to Swiss-Prot)
  • Protein family: type-B carboxylesterase/lipase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Ser-189 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
    • repressed during logarithmic growth (AbrB) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
    • GP951 (pnbA::cat), available in Stülke lab
    • GP955 (slrR-pnbA::cat), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed