TreA

Gene name	treA
Synonyms	treC
Essential	no
Product	phospho-alpha-glucosidase
Function	trehalose utilization
Gene expression levels in SubtiExpress: treA
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	65 kDa, 4.895
Gene length, protein length	1683 bp, 561 aa
Immediate neighbours	treP, treR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, PhoP regulon, TreR regulon

The gene

Basic information

• Locus tag: BSU07810

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Alpha,alpha-trehalose 6-phosphate + H₂O = D-glucose + D-glucose 6-phosphate (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 13 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P39795

• KEGG entry: [3]

• E.C. number: 3.2.1.93

Expression and regulation

• Operon: treP-treA-treR PubMed

• Expression browser: treA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by trehalose (TreR) PubMed
  • repressed by glucose (CcpA) PubMed
  • expressed under conditions of phosphate limitation (PhoP) PubMed

• Regulatory mechanism:
  • TreR: transcription repression PubMed
  • CcpA: transcription repression PubMed
  • PhoP: transcription activation PubMed

• Additional information:

Biological materials

• Mutant: GP1118 (spc), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Hiromi Ujiie, Tomoko Matsutani, Hisashi Tomatsu, Ai Fujihara, Chisato Ushida, Yasuhiko Miwa, Yasutaro Fujita, Hyouta Himeno, Akira Muto
Trans-translation is involved in the CcpA-dependent tagging and degradation of TreP in Bacillus subtilis.
J Biochem: 2009, 145(1);59-66
[PubMed:18977770] [WorldCat.org] [DOI] (I p)

F Schöck, M K Dahl
Analysis of DNA flanking the treA gene of Bacillus subtilis reveals genes encoding a putative specific enzyme IITre and a potential regulator of the trehalose operon.
Gene: 1996, 175(1-2);59-63
[PubMed:8917076] [WorldCat.org] [DOI] (P p)

F Schöck, M K Dahl
Expression of the tre operon of Bacillus subtilis 168 is regulated by the repressor TreR.
J Bacteriol: 1996, 178(15);4576-81
[PubMed:8755887] [WorldCat.org] [DOI] (P p)

S Gotsche, M K Dahl
Purification and characterization of the phospho-alpha(1,1)glucosidase (TreA) of Bacillus subtilis 168.
J Bacteriol: 1995, 177(10);2721-6
[PubMed:7751281] [WorldCat.org] [DOI] (P p)

C Helfert, S Gotsche, M K Dahl
Cleavage of trehalose-phosphate in Bacillus subtilis is catalysed by a phospho-alpha-(1-1)-glucosidase encoded by the treA gene.
Mol Microbiol: 1995, 16(1);111-120
[PubMed:7651129] [WorldCat.org] [DOI] (P p)