ZnuB

Gene name	znuB
Synonyms	yceA
Essential	no
Product	ABC transporter for zinc (permease)
Function	zinc uptake
Gene expression levels in SubtiExpress: znuB
Interactions involving this protein in SubtInteract: ZnuB
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	30 kDa, 10.375
Gene length, protein length	840 bp, 280 aa
Immediate neighbours	znuC, yceB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, trace metal homeostasis (Cu, Zn, Ni, Mn, Mo), membrane proteins

This gene is a member of the following regulons

Zur regulon

The gene

Basic information

• Locus tag: BSU02870

Phenotypes of a mutant

• • reduced genetic competence, can be rescued by the addition of excess zinc PubMed
  • reduced expression of the comFA-comFB-comFC-yvyF-flgM-yvyG-flgK-flgL operon PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ABC-3 integral membrane protein family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: ZnuA-ZnuB-ZnuC PubMed

• Localization: cell membrane PubMed

Database entries

• Structure:

• UniProt: O34610

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: znuA-znuC-znuB PubMed

• Expression browser: znuB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by zinc starvation (Zur) PubMed

• Regulatory mechanism:
  • Zur: transcription repression in the presence of zinc PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Mitsuo Ogura
ZnuABC and ZosA zinc transporters are differently involved in competence development in Bacillus subtilis.
J Biochem: 2011, 150(6);615-25
[PubMed:21813502] [WorldCat.org] [DOI] (I p)

Ahmed Gaballa, Tao Wang, Rick W Ye, John D Helmann
Functional analysis of the Bacillus subtilis Zur regulon.
J Bacteriol: 2002, 184(23);6508-14
[PubMed:12426338] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)

A Gaballa, J D Helmann
Identification of a zinc-specific metalloregulatory protein, Zur, controlling zinc transport operons in Bacillus subtilis.
J Bacteriol: 1998, 180(22);5815-21
[PubMed:9811636] [WorldCat.org] [DOI] (P p)