SkfE

Gene name	skfE
Synonyms	ybdA
Essential	no
Product	ABC transporter (binding protein)
Function	export of the spore killing factor SkfA
Gene expression levels in SubtiExpress: skfE
Interactions involving this protein in SubtInteract: SkfE
MW, pI	26 kDa, 6.34
Gene length, protein length	717 bp, 239 aa
Immediate neighbours	skfC, skfF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, toxins, antitoxins and immunity against toxins, membrane proteins

This gene is a member of the following regulons

AbrB regulon, PhoP regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU01950

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ABC transporter domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SkfE-SkfF PubMed

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O31427

• KEGG entry: [2]

• E.C. number: 3.6.3.25

Expression and regulation

• Operon: skfA-skfB-skfC-skfE-skfF-skfG-skfH PubMed

• Expression browser: skfE PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions that trigger sporulation (Spo0A) PubMed
  • repressed during logrithmic growth (AbrB) PubMed
  • induced by phosphate starvation (PhoP) PubMed

• Regulatory mechanism:
  • Spo0A: transcription activation PubMed
  • AbrB: transcription repression PubMed
  • PhoP: transcription activation PubMed

• Additional information: Northern blotting during during phosphate limitation showed an intense 0.25 kb skfA-specific transcript, and a weaker 6.5 kb skfA-skfB-skfC-skfE-skfF-skfG-skfH transcript. PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

José Eduardo González-Pastor
Cannibalism: a social behavior in sporulating Bacillus subtilis.
FEMS Microbiol Rev: 2011, 35(3);415-24
[PubMed:20955377] [WorldCat.org] [DOI] (I p)