SigH

Gene name	sigH
Synonyms	spo0H
Essential	no
Product	RNA polymerase sigma factor SigH
Function	transcription of early stationary phase genes (sporulation, competence)
Gene expression levels in SubtiExpress: sigH
Interactions involving this protein in SubtInteract: SigH
MW, pI	25 kDa, 5.146
Gene length, protein length	654 bp, 218 aa
Immediate neighbours	yacP, rpmGB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription, sigma factors and their control

This gene is a member of the following regulons

AbrB regulon

The gene

Basic information

• Locus tag: BSU00980

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: sigma-70 factor family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • (RpoB-RpoC)-SigH

• Localization:

Database entries

• Structure:

• UniProt: P17869

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sigH (DBTBS)

• Expression browser: sigH PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed during logrithmic growth (AbrB) PubMed

• Regulatory mechanism:
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Charles Moran, Emory University, NC, USA homepage

References

Reviews

Michiel J L de Hoon, Patrick Eichenberger, Dennis Vitkup
Hierarchical evolution of the bacterial sporulation network.
Curr Biol: 2010, 20(17);R735-45
[PubMed:20833318] [WorldCat.org] [DOI] (I p)

The SigH regulon

Robert A Britton, Patrick Eichenberger, Jose Eduardo Gonzalez-Pastor, Paul Fawcett, Rita Monson, Richard Losick, Alan D Grossman
Genome-wide analysis of the stationary-phase sigma factor (sigma-H) regulon of Bacillus subtilis.
J Bacteriol: 2002, 184(17);4881-90
[PubMed:12169614] [WorldCat.org] [DOI] (P p)

Original Publications

Imke G de Jong, Jan-Willem Veening, Oscar P Kuipers
Heterochronic phosphorelay gene expression as a source of heterogeneity in Bacillus subtilis spore formation.
J Bacteriol: 2010, 192(8);2053-67
[PubMed:20154131] [WorldCat.org] [DOI] (I p)

C Eymann, M Hecker
Induction of sigma(B)-dependent general stress genes by amino acid starvation in a spo0H mutant of Bacillus subtilis.
FEMS Microbiol Lett: 2001, 199(2);221-7
[PubMed:11377871] [WorldCat.org] [DOI] (P p)

C M Buckner, C P Moran
A region in Bacillus subtilis sigmaH required for Spo0A-dependent promoter activity.
J Bacteriol: 1998, 180(18);4987-90
[PubMed:9733708] [WorldCat.org] [DOI] (P p)

P A Levin, R Losick
Transcription factor Spo0A switches the localization of the cell division protein FtsZ from a medial to a bipolar pattern in Bacillus subtilis.
Genes Dev: 1996, 10(4);478-88
[PubMed:8600030] [WorldCat.org] [DOI] (P p)

M A Strauch
Delineation of AbrB-binding sites on the Bacillus subtilis spo0H, kinB, ftsAZ, and pbpE promoters and use of a derived homology to identify a previously unsuspected binding site in the bsuB1 methylase promote.
J Bacteriol: 1995, 177(23);6999-7002
[PubMed:7592498] [WorldCat.org] [DOI] (P p)

T Bird, D Burbulys, J J Wu, M A Strauch, J A Hoch, G B Spiegelman
The effect of supercoiling on the in vitro transcription of the spoIIA operon from Bacillus subtilis.
Biochimie: 1992, 74(7-8);627-34
[PubMed:1391042] [WorldCat.org] [DOI] (P p)

J Healy, J Weir, I Smith, R Losick
Post-transcriptional control of a sporulation regulatory gene encoding transcription factor sigma H in Bacillus subtilis.
Mol Microbiol: 1991, 5(2);477-87
[PubMed:1904128] [WorldCat.org] [DOI] (P p)

J Weir, M Predich, E Dubnau, G Nair, I Smith
Regulation of spo0H, a gene coding for the Bacillus subtilis sigma H factor.
J Bacteriol: 1991, 173(2);521-9
[PubMed:1898930] [WorldCat.org] [DOI] (P p)

D Daniels, P Zuber, R Losick
Two amino acids in an RNA polymerase sigma factor involved in the recognition of adjacent base pairs in the -10 region of a cognate promoter.
Proc Natl Acad Sci U S A: 1990, 87(20);8075-9
[PubMed:2122453] [WorldCat.org] [DOI] (P p)

U Bai, M Lewandoski, E Dubnau, I Smith
Temporal regulation of the Bacillus subtilis early sporulation gene spo0F.
J Bacteriol: 1990, 172(9);5432-9
[PubMed:2118512] [WorldCat.org] [DOI] (P p)

K M Tatti, H L Carter, A Moir, C P Moran
Sigma H-directed transcription of citG in Bacillus subtilis.
J Bacteriol: 1989, 171(11);5928-32
[PubMed:2509422] [WorldCat.org] [DOI] (P p)

K J Jaacks, J Healy, R Losick, A D Grossman
Identification and characterization of genes controlled by the sporulation-regulatory gene spo0H in Bacillus subtilis.
J Bacteriol: 1989, 171(8);4121-9
[PubMed:2502532] [WorldCat.org] [DOI] (P p)

P Zuber, J Healy, H L Carter, S Cutting, C P Moran, R Losick
Mutation changing the specificity of an RNA polymerase sigma factor.
J Mol Biol: 1989, 206(4);605-14
[PubMed:2500529] [WorldCat.org] [DOI] (P p)

H L Carter, L F Wang, R H Doi, C P Moran
rpoD operon promoter used by sigma H-RNA polymerase in Bacillus subtilis.
J Bacteriol: 1988, 170(4);1617-21
[PubMed:3127379] [WorldCat.org] [DOI] (P p)

C Ray, M Igo, W Shafer, R Losick, C P Moran
Suppression of ctc promoter mutations in Bacillus subtilis.
J Bacteriol: 1988, 170(2);900-7
[PubMed:3123466] [WorldCat.org] [DOI] (P p)

P Zuber, R Losick
spoOH: a developmental regulatory gene for promoter utilization in Bacillus subtilis.
Cold Spring Harb Symp Quant Biol: 1985, 50;483-8
[PubMed:3007003] [WorldCat.org] [DOI] (P p)

W C Johnson, C P Moran, R Losick
Two RNA polymerase sigma factors from Bacillus subtilis discriminate between overlapping promoters for a developmentally regulated gene.
Nature: 1983, 302(5911);800-4
[PubMed:6405278] [WorldCat.org] [DOI] (P p)