Difference between revisions of "PheT"

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|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pheT_2927008_2929422_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:pheT_expression.png|500px]]
 
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Revision as of 10:35, 20 April 2012

  • Description: phenylalanyl-tRNA synthetase (beta subunit)

Gene name pheT
Synonyms
Essential yes PubMed
Product phenylalanyl-tRNA synthetase (beta subunit)
Function translation
Interactions involving this protein in SubtInteract: PheT
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging
MW, pI 87 kDa, 4.864
Gene length, protein length 2412 bp, 804 aa
Immediate neighbours rnhC, pheS
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PheT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PheT expression.png




























Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box

The gene

Basic information

  • Locus tag: BSU28630

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe) (according to Swiss-Prot)
  • Protein family: Type 1 subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1PYS (from Thermus thermophilus hb8 mutant, 37% identity, 56% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

A A Brakhage, M Wozny, H Putzer
Structure and nucleotide sequence of the Bacillus subtilis phenylalanyl-tRNA synthetase genes.
Biochimie: 1990, 72(10);725-34
[PubMed:2127701] [WorldCat.org] [DOI] (P p)