Abf2

• Description: alpha-L-arabinofuranosidase
  
  

Gene name	abf2
Synonyms	xsa
Essential	no
Product	alpha-L-arabinofuranosidase
Function	arabinan degradation
MW, pI	56 kDa, 5.329
Gene length, protein length	1485 bp, 495 aa
Immediate neighbours	trxA, etfA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

AraR regulon

The gene

Basic information

• Locus tag: BSU28510

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 51 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P94552

• KEGG entry: [3]

• E.C. number: 3.2.1.55

Expression and regulation

• Operon: abf2 PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by arabinose (AraR) PubMed

• Regulatory mechanism:
  • AraR: transcription repression PubMed

• Additional information:
  • A ncRNA is predicted between trxA and abf2 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

José Manuel Inácio, Isabel Lopes Correia, Isabel de Sá-Nogueira
Two distinct arabinofuranosidases contribute to arabino-oligosaccharide degradation in Bacillus subtilis.
Microbiology (Reading): 2008, 154(Pt 9);2719-2729
[PubMed:18757805] [WorldCat.org] [DOI] (P p)

Maria Paiva Raposo, José Manuel Inácio, Luís Jaime Mota, Isabel de Sá-Nogueira
Transcriptional regulation of genes encoding arabinan-degrading enzymes in Bacillus subtilis.
J Bacteriol: 2004, 186(5);1287-96
[PubMed:14973026] [WorldCat.org] [DOI] (P p)