CitM

• Description: Mg2+-citrate transporter
  
  

Gene name	citM
Synonyms	yflO
Essential	no
Product	Mg2+-citrate transporter
Function	citrate uptake
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism, Metal ion homeostasis
MW, pI	45 kDa, 6.227
Gene length, protein length	1299 bp, 433 aa
Immediate neighbours	yflP, yflN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transporters/ other, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

CcpA regulon, CitT regulon

The gene

Basic information

• Locus tag: BSU07610

Phenotypes of a mutant

no growth with citrate as sole carbon source PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: View classification (according to Swiss-Prot)

• Paralogous protein(s): CitH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P55069

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: citM-yflN PubMed

• Sigma factor:

• Regulation:
  • induced by citrate (CitT) PubMed
  • repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • CitT: transcription activation PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Guillermo D Repizo, Víctor S Blancato, Pablo D Sender, Juke Lolkema, Christian Magni
Catabolite repression of the citST two-component system in Bacillus subtilis.
FEMS Microbiol Lett: 2006, 260(2);224-31
[PubMed:16842348] [WorldCat.org] [DOI] (P p)

Jessica B Warner, Juke S Lolkema
A Crh-specific function in carbon catabolite repression in Bacillus subtilis.
FEMS Microbiol Lett: 2003, 220(2);277-80
[PubMed:12670692] [WorldCat.org] [DOI] (P p)

Jessica B Warner, Christian Magni, Juke S Lolkema
CcpA-independent regulation of expression of the Mg2+ -citrate transporter gene citM by arginine metabolism in Bacillus subtilis.
J Bacteriol: 2003, 185(3);854-9
[PubMed:12533460] [WorldCat.org] [DOI] (P p)

Jessica B Warner, Juke S Lolkema
Growth of Bacillus subtilis on citrate and isocitrate is supported by the Mg2+-citrate transporter CitM.
Microbiology (Reading): 2002, 148(Pt 11);3405-3412
[PubMed:12427932] [WorldCat.org] [DOI] (P p)

Bastiaan P Krom, Henry Huttinga, Jessica B Warner, Juke S Lolkema
Impact of the Mg(2+)-citrate transporter CitM on heavy metal toxicity in Bacillus subtilis.
Arch Microbiol: 2002, 178(5);370-5
[PubMed:12375105] [WorldCat.org] [DOI] (P p)

H Li, A M Pajor
Functional characterization of CitM, the Mg2+-citrate transporter.
J Membr Biol: 2002, 185(1);9-16
[PubMed:11891560] [WorldCat.org] [DOI] (P p)

B P Krom, J B Warner, W N Konings, J S Lolkema
Complementary metal ion specificity of the metal-citrate transporters CitM and CitH of Bacillus subtilis.
J Bacteriol: 2000, 182(22);6374-81
[PubMed:11053381] [WorldCat.org] [DOI] (P p)

J B Warner, B P Krom, C Magni, W N Konings, J S Lolkema
Catabolite repression and induction of the Mg(2+)-citrate transporter CitM of Bacillus subtilis.
J Bacteriol: 2000, 182(21);6099-105
[PubMed:11029430] [WorldCat.org] [DOI] (P p)

H Yamamoto, M Murata, J Sekiguchi
The CitST two-component system regulates the expression of the Mg-citrate transporter in Bacillus subtilis.
Mol Microbiol: 2000, 37(4);898-912
[PubMed:10972810] [WorldCat.org] [DOI] (P p)

A Boorsma, M E van der Rest, J S Lolkema, W N Konings
Secondary transporters for citrate and the Mg(2+)-citrate complex in Bacillus subtilis are homologous proteins.
J Bacteriol: 1996, 178(21);6216-22
[PubMed:8892821] [WorldCat.org] [DOI] (P p)

P Carlsson, L Hederstedt
Bacillus subtilis citM, the structural gene for dihydrolipoamide transsuccinylase: cloning and expression in Escherichia coli.
Gene: 1987, 61(2);217-24
[PubMed:3127276] [WorldCat.org] [DOI] (P p)