GroEL

• Description: chaperonin and co-repressor for HrcA
  
  

Gene name	groEL
Synonyms
Essential	yes PubMed
Product	chaperonin, co-repressor for HrcA
Function	protein folding and re-folding
Interactions involving this protein in SubtInteract: GroEL
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	57 kDa, 4.531
Gene length, protein length	1632 bp, 544 aa
Immediate neighbours	groES, ydiM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

chaperones/ protein folding, heat shock proteins, essential genes, phosphoproteins

This gene is a member of the following regulons

HrcA regulon

The gene

Basic information

• Locus tag: BSU06030

Expression

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: acts as co-repressor for HrcA

• Protein family: chaperonin (HSP60) family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on ser/ thr/ tyr PubMed, PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions: HrcA-GroEL, GroEL-GroES

• Localization:
  • cytoplasm (according to Swiss-Prot)
  • recruited to the membrane after ethanol stress PubMed

Database entries

• Structure:

• UniProt: P28598

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: groES-groEL PubMed

• Sigma factor: SigA PubMed

• Regulation: induced by heat shock (HrcA) PubMed

• Regulatory mechanism: HrcA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:
  • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP1000 available in Stülke lab
  • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP1002 available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Wolfgang Schumann, Bayreuth University, Germany Homepage