Lip

• Description: extracellular lipase
  
  

Gene name	lip
Synonyms	lipA
Essential	no
Product	extracellular lipase
Function	lipid degradation
MW, pI	22 kDa, 10.059
Gene length, protein length	636 bp, 212 aa
Immediate neighbours	ansZ, yczC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of lipids

This gene is a member of the following regulons

AbrB regulon

The gene

Basic information

• Locus tag: BSU02700

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): LipB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2QXT

• UniProt: P37957

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: lip PubMed

• Sigma factor:

• Regulation:
  • repressed during vegetative growth (AbrB) PubMed

• Regulatory mechanism:
  • AbrB: transcription repression PubMed

• Additional information:
  • the amount of the mRNA is substantially decreased upon depletion of RNase Y PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Wojciech Augustyniak, Agnieszka A Brzezinska, Tjaard Pijning, Hans Wienk, Rolf Boelens, Bauke W Dijkstra, Manfred T Reetz
Biophysical characterization of mutants of Bacillus subtilis lipase evolved for thermostability: factors contributing to increased activity retention.
Protein Sci: 2012, 21(4);487-97
[PubMed:22267088] [WorldCat.org] [DOI] (I p)

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947