Difference between revisions of "HypR"

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m (moved YybR to HypR: 22238377)
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* '''Description:''' MarR/DUF24-family transcription regulator, positively controls the nitroreductase gene ''[[yfkO]]'' (hypO) in response to disulfide stress <br/><br/>
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* '''Description:''' MarR/DUF24-family transcription regulator, positively controls the nitroreductase gene ''[[hypO]]'' in response to disulfide stress <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
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| ''yybR'' (hypR)
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| ''hypR''  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''hypR''
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yybR''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
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|style="background:#ABCDEF;" align="center"| '''Product''' || MarR/DUF24-family transcription regulator HypR   
 
|style="background:#ABCDEF;" align="center"| '''Product''' || MarR/DUF24-family transcription regulator HypR   
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || positively controls nitroreductase gene yfkO (hypO) in response to disulfide stress (diamide, NaOCl)
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|style="background:#ABCDEF;" align="center"|'''Function''' || control of the nitroreductase gene ''[[hypO]]'' in response to disulfide stress (diamide, NaOCl)
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 8.415   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 8.415   
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= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
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{{SubtiWiki regulon|[[YybR (HypR) regulon]]}}
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{{SubtiWiki regulon|[[HypR regulon]]}}
  
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= The [[YybR (HypR) regulon]]:=
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= The [[HypR regulon]]:=
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* ''[[yybR]]'' (hypR), ''[[yfkO]]'' (hypO)
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* ''[[hypR]]'', ''[[hypO]]''  
  
 
=The gene=
 
=The gene=
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=== Additional information===
 
=== Additional information===
−
hypR autoregulated by disulfide stress
+
''hypR'' is autoregulated by disulfide stress
  
  
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* '''Protein family:''' MarR/DUF24-family regulator
 
* '''Protein family:''' MarR/DUF24-family regulator
  
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* '''Paralogous protein(s):''' YdeP,YkvN
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* '''Paralogous protein(s):''' [[YdeP]],[[YkvN]]
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
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* '''Kinetic information:''' Cys14 redox sensing Cys, has lower pKa of 6.36 {{PubMed|22238377}}  
 
* '''Kinetic information:''' Cys14 redox sensing Cys, has lower pKa of 6.36 {{PubMed|22238377}}  
  
−
* '''Domains:''' 5 alpha helices, 2 beta sheets, MarR-fold with wHTH motif, alpha4 major groove recognition helix, beta2 and 3 form the wing; alpha5 dimer interface {{PubMed|22238377}}  
+
* '''Domains:'''  
−
* '''Modification:''' oxidized to Cys14-Cys49' intersubunit disulfides by disulfide stress {{PubMed|22238377}}  
+
** 5 alpha helices, 2 beta sheets, MarR-fold with wHTH motif, alpha4 major groove recognition helix, beta2 and 3 form the wing; alpha5 dimer interface {{PubMed|22238377}}  
 +
 
 +
* '''Modification:'''  
 +
** oxidized to Cys14-Cys49' intersubunit disulfides by disulfide stress {{PubMed|22238377}}
 +
** Cys14 and Cys49' are about 8-9 Angström apart in reduced HypR-Dimer, oxidation moves the major groove recognition alpha4 helices of the HypR dimer about 4 Angstroem towards each other that leads to activation of HypR {{PubMed|22238377}}  
  
 
* '''Cofactor(s):'''
 
* '''Cofactor(s):'''
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* '''Structure:'''  
 
* '''Structure:'''  
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reduced HypRC14S dimer [http://www.pdb.org/pdb/explore.do?structureId=4A5N]
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** reduced HypRC14S dimer [http://www.pdb.org/pdb/explore.do?structureId=4A5N] {{PubMed|22238377}}
−
oxidized HypR C14-C49' intersubunit disulfide-linked dimer [http://www.pdb.org/pdb/explore.do?structureId=4A5M]
+
** oxidized HypR C14-C49' intersubunit disulfide-linked dimer [http://www.pdb.org/pdb/explore.do?structureId=4A5M] {{PubMed|22238377}}
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P37486 P37486]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P37486 P37486]
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=Expression and regulation=
 
=Expression and regulation=
  
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* '''Operon:''' ''yybR'' (hypR)(according to [http://dbtbs.hgc.jp/COG/prom/yybR.html DBTBS])
+
* '''Operon:''' ''hypR'' (according to [http://dbtbs.hgc.jp/COG/prom/yybR.html DBTBS])
  
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
  
−
* '''Regulation:''' activated by disulfide stress conditions (diamide, NaOCl) in vivo and in vitro {{PubMed|22238377}}  
+
* '''Regulation:'''  
 +
** activated by disulfide stress conditions (diamide, NaOCl) ''in vivo'' and ''in vitro '' {{PubMed|22238377}}  
  
−
* '''Regulatory mechanism:''' redox-controlled by Cys14-Cys49' intersubunit disulfide formation by diamide and NaOCl in vitro and vivo {{PubMed|22238377}}  
+
* '''Regulatory mechanism:'''
 +
** redox-controlled by Cys14-Cys49' intersubunit disulfide formation by diamide and NaOCl ''in vitro'' and ''in vivo'' {{PubMed|22238377}}  
  
−
* '''Additional information:''' Cys14 and Cys49' are about 8-9 Angstroem apart in reduced HypR-Dimer, oxidation moves the major groove recognition alpha4 helices of the HypR dimer about 4 Angstroem towards each other that leads to activation of HypR {{PubMed|22238377}}
+
* '''Additional information:'''  
  
 
=Biological materials =
 
=Biological materials =
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=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
  
−
[[Haike Antelmann]],University of Greifswald, Germany
+
* [[Haike Antelmann]],University of Greifswald, Germany
  
 
=Your additional remarks=
 
=Your additional remarks=
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=Original articles=
 
=Original articles=
−
<pubmed>22238377</pubmed>
+
'''Additional publications:''' {{PubMed|22238377}}
 +
<pubmed></pubmed>
  
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 19:32, 16 January 2012

  • Description: MarR/DUF24-family transcription regulator, positively controls the nitroreductase gene hypO in response to disulfide stress

Gene name hypR
Synonyms yybR
Essential no
Product MarR/DUF24-family transcription regulator HypR
Function control of the nitroreductase gene hypO in response to disulfide stress (diamide, NaOCl)
MW, pI 14 kDa, 8.415
Gene length, protein length 375 bp, 125 aa
Immediate neighbours cotF, ppaC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YybR context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

transcription factors and their control, resistance against oxidative and electrophile stress

This gene is a member of the following regulons

This gene is a member of the following regulons

HypR regulon

The HypR regulon:

The gene

Basic information

  • Locus tag: BSU40540

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

hypR is autoregulated by disulfide stress


The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: MarR/DUF24-family regulator

Extended information on the protein

  • Kinetic information: Cys14 redox sensing Cys, has lower pKa of 6.36 PubMed
  • Domains:
    • 5 alpha helices, 2 beta sheets, MarR-fold with wHTH motif, alpha4 major groove recognition helix, beta2 and 3 form the wing; alpha5 dimer interface PubMed
  • Modification:
    • oxidized to Cys14-Cys49' intersubunit disulfides by disulfide stress PubMed
    • Cys14 and Cys49' are about 8-9 Angström apart in reduced HypR-Dimer, oxidation moves the major groove recognition alpha4 helices of the HypR dimer about 4 Angstroem towards each other that leads to activation of HypR PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasmic

Database entries

  • Structure:
    • reduced HypRC14S dimer [3] PubMed
    • oxidized HypR C14-C49' intersubunit disulfide-linked dimer [4] PubMed
  • KEGG entry: [5]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: hypR (according to DBTBS)
  • Regulation:
    • activated by disulfide stress conditions (diamide, NaOCl) in vivo and in vitro PubMed
  • Regulatory mechanism:
    • redox-controlled by Cys14-Cys49' intersubunit disulfide formation by diamide and NaOCl in vitro and in vivo PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Haike Antelmann, John D Helmann
Thiol-based redox switches and gene regulation.
Antioxid Redox Signal: 2011, 14(6);1049-63
[PubMed:20626317] [WorldCat.org] [DOI] (I p)


Original articles

Additional publications: PubMed

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