Difference between revisions of "MrpB"
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* '''Operon:''' ''[[mrpA]]-[[mrpB]]-[[mrpC]]-[[mrpD]]-[[mrpE]]-[[mrpF]]-[[mrpG]]'' {{PubMed|9274030,10198001}} | * '''Operon:''' ''[[mrpA]]-[[mrpB]]-[[mrpC]]-[[mrpD]]-[[mrpE]]-[[mrpF]]-[[mrpG]]'' {{PubMed|9274030,10198001}} | ||
| − | * '''[ | + | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mrpB_3248996_3249427_1 mrpB] {{PubMed|22383849}} |
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| + | * '''Sigma factor:''' | ||
* '''Regulation:''' | * '''Regulation:''' | ||
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* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| − | * '''Additional information:''' | + | * '''Additional information:''' |
=Biological materials = | =Biological materials = | ||
Revision as of 16:23, 16 April 2012
- Description: Na+/H+ antiporter subunit, multiple resistance and pH homeostasis
| Gene name | mrpB |
| Synonyms | yufU |
| Essential | yes PubMed |
| Product | Na+/H+ antiporter subunit |
| Function | sodium export |
| Interactions involving this protein in SubtInteract: MprB | |
| MW, pI | 15 kDa, 4.912 |
| Gene length, protein length | 429 bp, 143 aa |
| Immediate neighbours | mrpA, mrpC |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
transporters/ other, metal ion homeostasis (K, Na, Ca, Mg), essential genes, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU31610
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O05259
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- SM-GN9 (mrpB-spc), available in Anne Galinier's and Boris Görke's labs
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Yusuke Kajiyama, Masato Otagiri, Junichi Sekiguchi, Toshiaki Kudo, Saori Kosono
The MrpA, MrpB and MrpD subunits of the Mrp antiporter complex in Bacillus subtilis contain membrane-embedded and essential acidic residues.
Microbiology (Reading): 2009, 155(Pt 7);2137-2147
[PubMed:19389778]
[WorldCat.org]
[DOI]
(P p)
Yusuke Kajiyama, Masato Otagiri, Junichi Sekiguchi, Saori Kosono, Toshiaki Kudo
Complex formation by the mrpABCDEFG gene products, which constitute a principal Na+/H+ antiporter in Bacillus subtilis.
J Bacteriol: 2007, 189(20);7511-4
[PubMed:17693497]
[WorldCat.org]
[DOI]
(P p)
M Ito, A A Guffanti, W Wang, T A Krulwich
Effects of nonpolar mutations in each of the seven Bacillus subtilis mrp genes suggest complex interactions among the gene products in support of Na(+) and alkali but not cholate resistance.
J Bacteriol: 2000, 182(20);5663-70
[PubMed:11004162]
[WorldCat.org]
[DOI]
(P p)
M Ito, A A Guffanti, B Oudega, T A Krulwich
mrp, a multigene, multifunctional locus in Bacillus subtilis with roles in resistance to cholate and to Na+ and in pH homeostasis.
J Bacteriol: 1999, 181(8);2394-402
[PubMed:10198001]
[WorldCat.org]
[DOI]
(P p)
Bauke Oudega, Gregory Koningstein, Luísa Rodrigues, Maria de Sales Ramon, Helmut Hilbert, Andreas Düsterhöft, Thomas M Pohl, Thomas Weitzenegger
Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai).
Microbiology (Reading): 1997, 143 ( Pt 8);2769-2774
[PubMed:9274030]
[WorldCat.org]
[DOI]
(P p)
