Lgt

• Description: prolipoprotein diacylglyceryl transferase
  
  

Gene name	lgt
Synonyms	gerF, yvoC
Essential	no
Product	prolipoprotein diacylglyceryl transferase
Function	lipomodification of lipoproteins
Interactions involving this protein in SubtInteract: Lgt
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism, Protein secretion
MW, pI	30 kDa, 8.96
Gene length, protein length	807 bp, 269 aa
Immediate neighbours	yvoD, hprK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

protein modification, germination, membrane proteins

The gene

Basic information

• Locus tag: BSU34990

Phenotypes of a mutant

• reduced ability of spores to germinate with alanine due ti reduced activity of the GerAA-GerAB-GerAC germinant receptor PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: lgt family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GerAC-Lgt to fix GerAC to diacylglycerol in the membrane PubMed

• Localization: membrane associated PubMed

Database entries

• Structure:

• UniProt: O34752

• KEGG entry: [2]

• E.C. number: 2.4.99.-

Expression and regulation

• Operon: hprK-lgt-yvoD-yvoE-yvoF PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP851 (deletion of lgt-yvoD-yvoE-yvoF, replaced by aphA3), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

PubMed

Lgt in other organisms

Jérémy Pailler, Willy Aucher, Magali Pires, Nienke Buddelmeijer
Phosphatidylglycerol::prolipoprotein diacylglyceryl transferase (Lgt) of Escherichia coli has seven transmembrane segments, and its essential residues are embedded in the membrane.
J Bacteriol: 2012, 194(9);2142-51
[PubMed:22287519] [WorldCat.org] [DOI] (I p)