IseA

• Description: inhibits in vitro activity of cell wall endopeptidases LytE and LytF, inhibits cell separation
  
  

Gene name	iseA
Synonyms	yoeB
Essential	no
Product	inhibitor of autolysins
Function	protection against cell envelope stress
Interactions involving this protein in SubtInteract: IseA
MW, pI	19 kDa, 10.176
Gene length, protein length	543 bp, 181 aa
Immediate neighbours	yoeA, trnSL-Arg1
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

cell wall degradation/ turnover, membrane proteins

This gene is a member of the following regulons

WalR regulon

The gene

Basic information

• Locus tag: BSU18380

Expression

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • IseA-LytF (catalytic domain) PubMed

• Localization: cell membrane (according to Swiss-Prot), localized to cell separation sites

Database entries

• Structure:

• UniProt: O34841

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • repressed by WalR PubMed
  • induced by vancomycin PubMed

• Regulatory mechanism:
  • WalR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hiroki Yamamoto, Masayuki Hashimoto, Yuhei Higashitsuji, Hiroyuki Harada, Nozomi Hariyama, Lisa Takahashi, Tomoaki Iwashita, Seika Ooiwa, Junichi Sekiguchi
Post-translational control of vegetative cell separation enzymes through a direct interaction with specific inhibitor IseA in Bacillus subtilis.
Mol Microbiol: 2008, 70(1);168-82
[PubMed:18761694] [WorldCat.org] [DOI] (I p)

Paola Bisicchia, David Noone, Efthimia Lioliou, Alistair Howell, Sarah Quigley, Thomas Jensen, Hanne Jarmer, Kevin M Devine
The essential YycFG two-component system controls cell wall metabolism in Bacillus subtilis.
Mol Microbiol: 2007, 65(1);180-200
[PubMed:17581128] [WorldCat.org] [DOI] (P p)

Letal I Salzberg, John D Helmann
An antibiotic-inducible cell wall-associated protein that protects Bacillus subtilis from autolysis.
J Bacteriol: 2007, 189(13);4671-80
[PubMed:17483219] [WorldCat.org] [DOI] (P p)