Csn

• Description: chitosanase
  
  

Gene name	csn
Synonyms
Essential	no
Product	chitosanase
Function	chitin degradation
MW, pI	31 kDa, 8.892
Gene length, protein length	831 bp, 277 aa
Immediate neighbours	yraM, yraL
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

AbrB regulon

The gene

Basic information

• Locus tag: BSU26890

Expression

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Endohydrolysis of beta-(1->4)-linkages between D-glucosamine residues in a partly acetylated chitosan (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 46 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • extracellular (signal peptide), major constituent of the secretome PubMed

Database entries

• Structure:

• UniProt: O07921

• KEGG entry: [2]

• E.C. number: 3.2.1.132

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Chulhong Oh, Mahanama De Zoysa, Do-Hyung Kang, Youngdeuk Lee, Ilson Whang, Chamilani Nikapitiya, Soo-Jin Heo, Kon-Tak Yoon, Abu Affan, Jehee Lee
Isolation, purification, and enzymatic characterization of extracellular chitosanase from marine bacterium Bacillus subtilis CH2.
J Microbiol Biotechnol: 2011, 21(10);1021-5
[PubMed:22031025] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Luis A Rivas, Vı Ctor Parro, Mercedes Moreno-Paz, Rafael P Mellado
The Bacillus subtilis 168 csn gene encodes a chitosanase with similar properties to a streptomyces enzyme.
Microbiology (Reading): 2000, 146 ( Pt 11);2929-2936
[PubMed:11065371] [WorldCat.org] [DOI] (P p)