Difference between revisions of "DnaE"

From SubtiWiki
Jump to: navigation, search
Line 110: Line 110:
 
* '''Operon:''' ''[[dnaE]]'' (according to [http://dbtbs.hgc.jp/COG/prom/dnaE.html DBTBS])
 
* '''Operon:''' ''[[dnaE]]'' (according to [http://dbtbs.hgc.jp/COG/prom/dnaE.html DBTBS])
  
* '''[[Sigma factor]]:'''  
+
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=dnaE_2991269_2994616_-1 dnaE] {{PubMed|22383849}}
 +
 
 +
* '''Sigma factor:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  

Revision as of 15:09, 16 April 2012

  • Description: DNA polymerase III (alpha subunit), part of the replisome

Gene name dnaE
Synonyms
Essential yes PubMed
Product DNA polymerase III (alpha subunit)
Function DNA replication
Interactions involving this protein in SubtInteract: DnaE
MW, pI 125 kDa, 6.02
Gene length, protein length 3345 bp, 1115 aa
Immediate neighbours ytsJ, ytrH
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
DnaE context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

DNA replication, essential genes

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

  • Locus tag: BSU29230

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
  • Protein family: DnaE subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • forms foci, the foci dissappear after the formation of DNA mismatches in a MutS-dependent manner or after DNA replication arrest PubMed
    • cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed