BacG

• Description: short chain reductase involved in bacilysin synthesis
  
  

Gene name	ywfH
Synonyms	ipa-86r
Essential	no
Product	short chain reductase
Function	biosynthesis of the antibiotic bacilysin
MW, pI	27 kDa, 6.596
Gene length, protein length	777 bp, 259 aa
Immediate neighbours	ywfI, bacF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

miscellaneous metabolic pathways, biosynthesis of antibacterial compounds

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

• Locus tag: BSU37680

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: YwfH catalyzes the conjugate addition of hydride at the C4 olefinic terminus using NADH to yield the cyclohexenol- containing tetrahydro-4-hydroxyphenylpyruvate PubMed

• Protein family: short-chain dehydrogenases/reductases (SDR) family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P39644

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: ywfH PubMed

• Sigma factor:

• Regulation:
  • repressed by casamino acids PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: