Difference between revisions of "NupN"

From SubtiWiki
Jump to: navigation, search
m (moved YufN to NupN: 21926227)
Line 1: Line 1:
* '''Description:''' similar to lipoprotein <br/><br/>
+
* '''Description:''' lipoprotein, part of guanosine transporter <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
Line 10: Line 10:
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
+
|style="background:#ABCDEF;" align="center"| '''Product''' || lipoprotein, part of guanosine transporter
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
+
|style="background:#ABCDEF;" align="center"|'''Function''' || uptake of guanosine
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 4.82   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 4.82   
Line 33: Line 33:
 
= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
 
{{SubtiWiki category|[[membrane proteins]]}},
 
{{SubtiWiki category|[[membrane proteins]]}},
{{SubtiWiki category|[[proteins of unknown function]]}}
+
{{SubtiWiki category|[[biosynthesis/ acquisition of nucleotides]]}}
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
Line 45: Line 45:
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
* a ''[[nupN]] [[nupG]]'' double mutant is unable to utilize externally provided guanosine {{PubMed|21926227}}
  
 
=== Database entries ===
 
=== Database entries ===
Line 53: Line 54:
  
 
=== Additional information===
 
=== Additional information===
 
 
  
  
Line 96: Line 95:
  
 
=Expression and regulation=
 
=Expression and regulation=
 +
* '''Operon:''' ''[[nupN]]-[[nupO]]-[[nupP]]-[[nupQ]]'' {{PubMed|21926227}}
  
* '''Operon:'''
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|21926227}}
 
 
* '''[[Sigma factor]]:'''  
 
  
 
* '''Regulation:'''
 
* '''Regulation:'''
** repressed during growth in the presence of branched chain amino acids ([[CodY]]) {{PubMed|12618455,21699902}}
+
** repressed during growth in the presence of branched chain amino acids ([[CodY]]) {{PubMed|12618455,21699902,21926227}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
** [[CodY]]: transcription repression {{PubMed|12618455,21699902}}
+
** [[CodY]]: transcription repression {{PubMed|12618455,21699902,21926227}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''
Line 129: Line 127:
 
=References=
 
=References=
  
<pubmed>12618455,18763711, 20935095 21699902 </pubmed>
+
<pubmed>12618455,18763711, 20935095 21699902 21926227</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 16:39, 20 September 2011

  • Description: lipoprotein, part of guanosine transporter

Gene name yufN
Synonyms
Essential no
Product lipoprotein, part of guanosine transporter
Function uptake of guanosine
MW, pI 37 kDa, 4.82
Gene length, protein length 1050 bp, 350 aa
Immediate neighbours malR, yufO
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YufN context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

membrane proteins, biosynthesis/ acquisition of nucleotides

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

  • Locus tag: BSU31540

Phenotypes of a mutant

  • a nupN nupG double mutant is unable to utilize externally provided guanosine PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: BMP lipoprotein family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot), membrane associated PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Boris R Belitsky, Abraham L Sonenshein
CodY-mediated regulation of guanosine uptake in Bacillus subtilis.
J Bacteriol: 2011, 193(22);6276-87
[PubMed:21926227] [WorldCat.org] [DOI] (I p)

Boris R Belitsky, Abraham L Sonenshein
Roadblock repression of transcription by Bacillus subtilis CodY.
J Mol Biol: 2011, 411(4);729-43
[PubMed:21699902] [WorldCat.org] [DOI] (I p)

Shaun R Brinsmade, Roelco J Kleijn, Uwe Sauer, Abraham L Sonenshein
Regulation of CodY activity through modulation of intracellular branched-chain amino acid pools.
J Bacteriol: 2010, 192(24);6357-68
[PubMed:20935095] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)