Difference between revisions of "MutTA"

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(Basic information/ Evolution)
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* '''Description:''' error prevention oxidized guanine system, confers protection against oxidative stress to vegetative cells <br/><br/>
+
* '''Description:''' error prevention oxidized guanine system, confers protection against oxidative stress to vegetative cells, moreover the protein has RNA pyrophosphohydrolase activity <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|''mutTA''
 
|''mutTA''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ytkD''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ytkD'', ''rppH''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || 8-oxo-dGTPase (antimutator)  
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|style="background:#ABCDEF;" align="center"| '''Product''' || 8-oxo-dGTPase (antimutator), RNA pyrophosphohydrolase
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || DNA repair
+
|style="background:#ABCDEF;" align="center"|'''Function''' || DNA repair, RNA degradation
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 18 kDa, 5.952   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 18 kDa, 5.952   
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' nucleoside triphosphate + 2H<sub>2</sub>O = nucleoside monophosphate + 2 phosphate (according to Swiss-Prot)
+
* '''Catalyzed reaction/ biological activity:'''  
 +
** removal of pyrophosphate from the 5' end of mRNAs to make the RNA accessible for degradation by [[RNases]] {{PubMed|21925382}}
  
 
* '''Protein family:''' [[Nudix hydrolase]] family (according to Swiss-Prot)
 
* '''Protein family:''' [[Nudix hydrolase]] family (according to Swiss-Prot)
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=References=
 
=References=
  
<pubmed>16513759,16497325, 14761999, 19011023 9387221 15576788 </pubmed>
+
<pubmed>16513759,16497325, 14761999, 19011023 9387221 15576788 21925382 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 15:55, 20 September 2011

  • Description: error prevention oxidized guanine system, confers protection against oxidative stress to vegetative cells, moreover the protein has RNA pyrophosphohydrolase activity

Gene name mutTA
Synonyms ytkD, rppH
Essential no
Product 8-oxo-dGTPase (antimutator), RNA pyrophosphohydrolase
Function DNA repair, RNA degradation
MW, pI 18 kDa, 5.952
Gene length, protein length 474 bp, 158 aa
Immediate neighbours ytlD, ytkC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YtkD context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

DNA repair/ recombination, sporulation proteins

This gene is a member of the following regulons

SigF regulon

The gene

Basic information

  • Locus tag: BSU30630

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • removal of pyrophosphate from the 5' end of mRNAs to make the RNA accessible for degradation by RNases PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed during logarithmic growth (SigA) and early during sporulation in the forespore (SigF) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Jamie Richards, Quansheng Liu, Olivier Pellegrini, Helena Celesnik, Shiyi Yao, David H Bechhofer, Ciarán Condon, Joel G Belasco
An RNA pyrophosphohydrolase triggers 5'-exonucleolytic degradation of mRNA in Bacillus subtilis.
Mol Cell: 2011, 43(6);940-9
[PubMed:21925382] [WorldCat.org] [DOI] (I p)

Luz E Vidales, Lluvia C Cárdenas, Eduardo Robleto, Ronald E Yasbin, Mario Pedraza-Reyes
Defects in the error prevention oxidized guanine system potentiate stationary-phase mutagenesis in Bacillus subtilis.
J Bacteriol: 2009, 191(2);506-13
[PubMed:19011023] [WorldCat.org] [DOI] (I p)

Francisco X Castellanos-Juárez, Carlos Alvarez-Alvarez, Ronald E Yasbin, Barbara Setlow, Peter Setlow, Mario Pedraza-Reyes
YtkD and MutT protect vegetative cells but not spores of Bacillus subtilis from oxidative stress.
J Bacteriol: 2006, 188(6);2285-9
[PubMed:16513759] [WorldCat.org] [DOI] (P p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Wenlian Xu, Candice R Jones, Christopher A Dunn, Maurice J Bessman
Gene ytkD of Bacillus subtilis encodes an atypical nucleoside triphosphatase member of the Nudix hydrolase superfamily.
J Bacteriol: 2004, 186(24);8380-4
[PubMed:15576788] [WorldCat.org] [DOI] (P p)

Martha I Ramírez, Francisco X Castellanos-Juárez, Ronald E Yasbin, Mario Pedraza-Reyes
The ytkD (mutTA) gene of Bacillus subtilis encodes a functional antimutator 8-Oxo-(dGTP/GTP)ase and is under dual control of sigma A and sigma F RNA polymerases.
J Bacteriol: 2004, 186(4);1050-9
[PubMed:14761999] [WorldCat.org] [DOI] (P p)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)